Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12110 | 5' | -56.6 | NC_003309.1 | + | 49468 | 0.73 | 0.292259 |
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Target: 5'- gCCGAGCACGCGcgaaugcCGAUUGCGaUGCa -3' miRNA: 3'- aGGCUCGUGUGCua-----GCUAGCGC-GCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 49854 | 0.68 | 0.545042 |
|
Target: 5'- uUCgGAGCACGu--UCu-UCGCGCGCGg -3' miRNA: 3'- -AGgCUCGUGUgcuAGcuAGCGCGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 50273 | 0.69 | 0.463372 |
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Target: 5'- gCCGAaacuGCGCucgaGAUCGcgaaagcugaucGUCGCGCGCGc -3' miRNA: 3'- aGGCU----CGUGug--CUAGC------------UAGCGCGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 52698 | 0.74 | 0.251557 |
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Target: 5'- cUCCGAGUugGCGAUUGG-CGUGUGUu -3' miRNA: 3'- -AGGCUCGugUGCUAGCUaGCGCGCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 53008 | 0.7 | 0.424993 |
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Target: 5'- cUCGuGCGCAUG-UUGAUCGCaGUGCGa -3' miRNA: 3'- aGGCuCGUGUGCuAGCUAGCG-CGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 53060 | 0.71 | 0.371149 |
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Target: 5'- aUCCucGCGCAUGA-CGAUUGCcGCGCa -3' miRNA: 3'- -AGGcuCGUGUGCUaGCUAGCG-CGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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