Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12110 | 5' | -56.6 | NC_003309.1 | + | 7667 | 1.08 | 0.000964 |
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Target: 5'- cUCCGAGCACACGAUCGAUCGCGCGCGc -3' miRNA: 3'- -AGGCUCGUGUGCUAGCUAGCGCGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 6769 | 0.67 | 0.587641 |
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Target: 5'- gCCuGGUACuuGAUCGucacggCGCGCGCa -3' miRNA: 3'- aGGcUCGUGugCUAGCua----GCGCGCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 6103 | 0.68 | 0.545042 |
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Target: 5'- gCCGgccAGCACAgCGAUCGAcucgaCGUGaCGCGg -3' miRNA: 3'- aGGC---UCGUGU-GCUAGCUa----GCGC-GCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 5566 | 0.66 | 0.63081 |
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Target: 5'- gUCUucGCGCGCGc-CGAUCGCG-GCGg -3' miRNA: 3'- -AGGcuCGUGUGCuaGCUAGCGCgCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 5158 | 0.66 | 0.684716 |
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Target: 5'- -gCGAGCuGCGCG-UCGc-CGCGCGCc -3' miRNA: 3'- agGCUCG-UGUGCuAGCuaGCGCGCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 3314 | 0.67 | 0.598401 |
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Target: 5'- gUCGAGCGCggaaaggcgcaGCGcGUCGAUCaGCGCuGCa -3' miRNA: 3'- aGGCUCGUG-----------UGC-UAGCUAG-CGCG-CGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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