Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12110 | 5' | -56.6 | NC_003309.1 | + | 3314 | 0.67 | 0.598401 |
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Target: 5'- gUCGAGCGCggaaaggcgcaGCGcGUCGAUCaGCGCuGCa -3' miRNA: 3'- aGGCUCGUG-----------UGC-UAGCUAG-CGCG-CGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 11861 | 0.67 | 0.607028 |
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Target: 5'- -gCGAGCucGCGCGucagauuGUCGAuguugguUCGCGCGCc -3' miRNA: 3'- agGCUCG--UGUGC-------UAGCU-------AGCGCGCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 24104 | 0.67 | 0.609187 |
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Target: 5'- gCCGGGaUACACGccgauAUCGGcacCGCGCGCc -3' miRNA: 3'- aGGCUC-GUGUGC-----UAGCUa--GCGCGCGc -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 16001 | 0.67 | 0.609187 |
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Target: 5'- -gCGAGCGCGaauUGGUCcGUCG-GCGCGg -3' miRNA: 3'- agGCUCGUGU---GCUAGcUAGCgCGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 17777 | 0.67 | 0.613508 |
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Target: 5'- -aCGGcGCACACgGAUCagccagccguuuuUCGCGCGCGg -3' miRNA: 3'- agGCU-CGUGUG-CUAGcu-----------AGCGCGCGC- -5' |
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| 12110 | 5' | -56.6 | NC_003309.1 | + | 22460 | 0.66 | 0.684716 |
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Target: 5'- aUCGuGCGCgGCGAUCGcacgcugccggcGUCGaGCGCGg -3' miRNA: 3'- aGGCuCGUG-UGCUAGC------------UAGCgCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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