Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12119 | 3' | -57.8 | NC_003309.1 | + | 9984 | 0.73 | 0.224465 |
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Target: 5'- uGAGCUgcgccgaacucuUGCCC-AUGCCGCGcGCGAUAg -3' miRNA: 3'- -CUCGA------------ACGGGcUGCGGCGCuUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 6509 | 0.66 | 0.531711 |
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Target: 5'- -cGCUUGCCgCG-CGCCcGgGAucACGACGc -3' miRNA: 3'- cuCGAACGG-GCuGCGG-CgCU--UGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 5331 | 0.68 | 0.440936 |
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Target: 5'- aGAGCggcagcgUGCgCG-CGCCgagcuuGCGAACGACGg -3' miRNA: 3'- -CUCGa------ACGgGCuGCGG------CGCUUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 5157 | 0.7 | 0.326993 |
|
Target: 5'- cGAGCUgcgcgucGCCgCGcgcCGCCGCGAGCGcACGu -3' miRNA: 3'- -CUCGAa------CGG-GCu--GCGGCGCUUGC-UGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 5064 | 0.68 | 0.440936 |
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Target: 5'- cGGGCgcgGCggCCGGUGCCGCG-ACGGCGg -3' miRNA: 3'- -CUCGaa-CG--GGCUGCGGCGCuUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 3847 | 0.69 | 0.385538 |
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Target: 5'- uGAGC--GCCUGAuuCGCCcauugcacgGCGAGCGACAu -3' miRNA: 3'- -CUCGaaCGGGCU--GCGG---------CGCUUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 3399 | 0.7 | 0.33494 |
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Target: 5'- gGAGCgacuggUGCUCGAUGuuGCuGAACGugGc -3' miRNA: 3'- -CUCGa-----ACGGGCUGCggCG-CUUGCugU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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