Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12139 | 3' | -55.2 | NC_003309.1 | + | 27798 | 0.66 | 0.635015 |
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Target: 5'- cGGACGAGUGGCGUGCccGCgaagAGCUcaAGUCu -3' miRNA: 3'- aUCUGCUCGUUGCGCG--CG----UCGG--UUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 47776 | 0.67 | 0.612523 |
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Target: 5'- --aGCGGGCAGCcuCGCGCAGCauuUCc -3' miRNA: 3'- aucUGCUCGUUGc-GCGCGUCGguuAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 22995 | 0.67 | 0.601299 |
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Target: 5'- --aGCGAGuCGACGCuuGCcGCCGAUCc -3' miRNA: 3'- aucUGCUC-GUUGCGcgCGuCGGUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 25999 | 0.67 | 0.578943 |
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Target: 5'- cGGGCGAaccgGCAauGCGUGCGUAGUC-GUCc -3' miRNA: 3'- aUCUGCU----CGU--UGCGCGCGUCGGuUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 47011 | 0.68 | 0.545773 |
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Target: 5'- -cGGCGAuuGGCGCGUcuucGCAGCCAacuuGUCg -3' miRNA: 3'- auCUGCUcgUUGCGCG----CGUCGGU----UAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 6148 | 0.68 | 0.513238 |
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Target: 5'- -uGACGGGCcGCGCGauuGCAGCU--UCg -3' miRNA: 3'- auCUGCUCGuUGCGCg--CGUCGGuuAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 22467 | 0.69 | 0.481525 |
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Target: 5'- -cGGCG-GCAuCGUGCGCGG-CGAUCg -3' miRNA: 3'- auCUGCuCGUuGCGCGCGUCgGUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 23764 | 1.09 | 0.000786 |
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Target: 5'- aUAGACGAGCAACGCGCGCAGCCAAUCg -3' miRNA: 3'- -AUCUGCUCGUUGCGCGCGUCGGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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