Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12140 | 5' | -55.7 | NC_003309.1 | + | 10705 | 0.73 | 0.270073 |
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Target: 5'- cUCGGCGCGcucgagcuccugcuGCgcgGCGGCCGUCuCGCGGg -3' miRNA: 3'- -AGCUGCGU--------------CGa--CGUCGGUAGuGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 16124 | 0.76 | 0.178138 |
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Target: 5'- gCGAUGCcGCUGCGcucGCaCAUCGCGCGAu -3' miRNA: 3'- aGCUGCGuCGACGU---CG-GUAGUGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 3952 | 0.76 | 0.173339 |
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Target: 5'- gUCGGCGCuccgauGCgGCAGCCAUCuGCGCGc -3' miRNA: 3'- -AGCUGCGu-----CGaCGUCGGUAG-UGUGCu -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 22473 | 0.76 | 0.173339 |
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Target: 5'- cCGACGCGGCggcaucgugcGCGGCgAUCGCACGc -3' miRNA: 3'- aGCUGCGUCGa---------CGUCGgUAGUGUGCu -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 45318 | 0.77 | 0.164088 |
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Target: 5'- gUUGAUGCauGGCaUGCAGCCAaCGCGCGAc -3' miRNA: 3'- -AGCUGCG--UCG-ACGUCGGUaGUGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 24007 | 1.1 | 0.000723 |
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Target: 5'- gUCGACGCAGCUGCAGCCAUCACACGAg -3' miRNA: 3'- -AGCUGCGUCGACGUCGGUAGUGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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