Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12140 | 5' | -55.7 | NC_003309.1 | + | 11581 | 0.67 | 0.576157 |
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Target: 5'- cCGACaGCAGCcGCAcGCCGUCGgcaGCGu -3' miRNA: 3'- aGCUG-CGUCGaCGU-CGGUAGUg--UGCu -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 19450 | 0.67 | 0.587139 |
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Target: 5'- gCGuguCGCGGC-GgAGCCGUCACcCGGg -3' miRNA: 3'- aGCu--GCGUCGaCgUCGGUAGUGuGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 13493 | 0.67 | 0.598158 |
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Target: 5'- gCGGCccGCuGGgUGUAGCCGUCGC-CGAa -3' miRNA: 3'- aGCUG--CG-UCgACGUCGGUAGUGuGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 22326 | 0.67 | 0.620276 |
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Target: 5'- cUCGACGCaAGCgccgGCcGCCAUgccaagcaGCGCGGc -3' miRNA: 3'- -AGCUGCG-UCGa---CGuCGGUAg-------UGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 20736 | 0.67 | 0.620276 |
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Target: 5'- cCGuCGCGGCUGaaccaCCAUCGCACc- -3' miRNA: 3'- aGCuGCGUCGACguc--GGUAGUGUGcu -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 15154 | 0.66 | 0.653506 |
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Target: 5'- cCGGCucCAGCUGCuggaugucuGCCGUUACACu- -3' miRNA: 3'- aGCUGc-GUCGACGu--------CGGUAGUGUGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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