Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12273 | 3' | -57.9 | NC_003309.1 | + | 31948 | 0.66 | 0.579989 |
Target: 5'- gCAGCGCAcgaacaguUCUU-CGCCGCccGCGGcCGGc -3' miRNA: 3'- -GUCGCGU--------AGAAcGCGGCGc-UGUC-GCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 10945 | 0.66 | 0.569267 |
Target: 5'- gAGCGcCGUC-UGCGCCGUcGCcGCGc -3' miRNA: 3'- gUCGC-GUAGaACGCGGCGcUGuCGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 3549 | 0.66 | 0.562857 |
Target: 5'- gCGGCGUAUCgcgacgacugauCGCCGCG-CAGCa- -3' miRNA: 3'- -GUCGCGUAGaac---------GCGGCGCuGUCGcc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 17759 | 0.66 | 0.558594 |
Target: 5'- cCAGC-CGUUUUuCGCgCGCGGCAGCu- -3' miRNA: 3'- -GUCGcGUAGAAcGCG-GCGCUGUCGcc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 40119 | 0.66 | 0.558594 |
Target: 5'- aCAGCGagaaCAUCggucGCGCCcaugcaauGCGGCAcGCGGc -3' miRNA: 3'- -GUCGC----GUAGaa--CGCGG--------CGCUGU-CGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 45757 | 0.66 | 0.54375 |
Target: 5'- uCGGCGCcugauguacGCGCCGguCGuACAGCGGg -3' miRNA: 3'- -GUCGCGuagaa----CGCGGC--GC-UGUCGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 12744 | 0.66 | 0.537427 |
Target: 5'- uCAGCGCcuccucggccAUC-UGCGCUGCGuucuGC-GCGGa -3' miRNA: 3'- -GUCGCG----------UAGaACGCGGCGC----UGuCGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 18727 | 0.67 | 0.526947 |
Target: 5'- gAGCGUgaucGUCUcGCcgGCUGCGGC-GCGGa -3' miRNA: 3'- gUCGCG----UAGAaCG--CGGCGCUGuCGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 43442 | 0.67 | 0.525903 |
Target: 5'- -cGCGCAgcc-GCGCCGCGAgauugaaCAGCa- -3' miRNA: 3'- guCGCGUagaaCGCGGCGCU-------GUCGcc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 27496 | 0.67 | 0.52486 |
Target: 5'- aAGCGCAUCcuugaagucgaGCGCgGCGAagaGGCGu -3' miRNA: 3'- gUCGCGUAGaa---------CGCGgCGCUg--UCGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 41041 | 0.67 | 0.523817 |
Target: 5'- cCAGCGUccGUCggGaCGCCGCcgcugcacaagcgcGACAGCGc -3' miRNA: 3'- -GUCGCG--UAGaaC-GCGGCG--------------CUGUCGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 37225 | 0.67 | 0.523817 |
Target: 5'- gCAGCGCGUCgaggacgagguaaagGCgaacGCCGCGAaGGuCGGa -3' miRNA: 3'- -GUCGCGUAGaa-------------CG----CGGCGCUgUC-GCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 18550 | 0.67 | 0.506225 |
Target: 5'- aCGGUGCG-CcUGCGCCuGCGACGuGCa- -3' miRNA: 3'- -GUCGCGUaGaACGCGG-CGCUGU-CGcc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 192 | 0.67 | 0.506225 |
Target: 5'- uCAGCGCGcgCUUGCcgggauuucccGCCGCGAUuuuccGCGc -3' miRNA: 3'- -GUCGCGUa-GAACG-----------CGGCGCUGu----CGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 6551 | 0.67 | 0.506225 |
Target: 5'- gUAGCGCGcCUUGCGUauCGCGGCgccAGCc- -3' miRNA: 3'- -GUCGCGUaGAACGCG--GCGCUG---UCGcc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 42199 | 0.67 | 0.506225 |
Target: 5'- gUAGUGgAUCUggGUGCCGCcauCAGCGa -3' miRNA: 3'- -GUCGCgUAGAa-CGCGGCGcu-GUCGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 7162 | 0.67 | 0.495994 |
Target: 5'- --aCGCAUCUgGuCGCCGauaccggccCGAUAGCGGa -3' miRNA: 3'- gucGCGUAGAaC-GCGGC---------GCUGUCGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 34566 | 0.67 | 0.485859 |
Target: 5'- gAGCGCGcagUGCGCCugguacGCGAgCAGCGu -3' miRNA: 3'- gUCGCGUagaACGCGG------CGCU-GUCGCc -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 53318 | 0.67 | 0.485859 |
Target: 5'- cCGGCGCAUC---CGCCGggcgggcaaCGACgGGCGGg -3' miRNA: 3'- -GUCGCGUAGaacGCGGC---------GCUG-UCGCC- -5' |
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12273 | 3' | -57.9 | NC_003309.1 | + | 31910 | 0.68 | 0.46589 |
Target: 5'- cCAGCGCGUCUUuuGCuCGUcGCuuGCGGa -3' miRNA: 3'- -GUCGCGUAGAAcgCG-GCGcUGu-CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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