Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12324 | 3' | -58.4 | NC_003324.1 | + | 19488 | 0.7 | 0.329515 |
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Target: 5'- gGGCGAUAGCGUCGGCgUcGGCGuGCUUc -3' miRNA: 3'- -UCGCUAUUGCGGUCGgA-CUGC-CGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 25590 | 0.7 | 0.321682 |
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Target: 5'- cGGCGGUGAUGacaUAGUCUGuCGGCCg- -3' miRNA: 3'- -UCGCUAUUGCg--GUCGGACuGCCGGgu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 11146 | 0.7 | 0.299025 |
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Target: 5'- cGCuu---CGCCGGUCUGGCGGCCg- -3' miRNA: 3'- uCGcuauuGCGGUCGGACUGCCGGgu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 30264 | 0.71 | 0.277623 |
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Target: 5'- cGGCGAUGAUGCgGGCCaagGcACGgucGCCCAa -3' miRNA: 3'- -UCGCUAUUGCGgUCGGa--C-UGC---CGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 9284 | 0.72 | 0.238511 |
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Target: 5'- cGCGuGUAACGCCAGCa--GCGGUCCu -3' miRNA: 3'- uCGC-UAUUGCGGUCGgacUGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 14608 | 0.75 | 0.160621 |
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Target: 5'- aGGCGGUAGCGUggCGGCCggagcuACGGCCCu -3' miRNA: 3'- -UCGCUAUUGCG--GUCGGac----UGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 5890 | 0.75 | 0.15634 |
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Target: 5'- uGGgGAUGACgGCCugcGCCuUGAUGGCCCGa -3' miRNA: 3'- -UCgCUAUUG-CGGu--CGG-ACUGCCGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 42758 | 0.75 | 0.148087 |
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Target: 5'- cAGCGGU--UGCCGGuCCUGGUGGCCCGg -3' miRNA: 3'- -UCGCUAuuGCGGUC-GGACUGCCGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 56147 | 0.75 | 0.147685 |
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Target: 5'- cGCGGguuCGCCAGCCUggaagccGACGGCCg- -3' miRNA: 3'- uCGCUauuGCGGUCGGA-------CUGCCGGgu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 4969 | 1.08 | 0.000558 |
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Target: 5'- aAGCGAUAACGCCAGCCUGACGGCCCAu -3' miRNA: 3'- -UCGCUAUUGCGGUCGGACUGCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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