Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12333 | 5' | -55.7 | NC_003324.1 | + | 38973 | 0.68 | 0.522082 |
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Target: 5'- cUUGCcGAGGCUGGUaaGA-GGCugaaccgccugcaguAUCGCCu -3' miRNA: 3'- -AACGcUUCCGACCA--CUaCCG---------------UAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 39655 | 0.68 | 0.550099 |
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Target: 5'- --cCGGAGGCUGGUGcUGaCGUggCGCCa -3' miRNA: 3'- aacGCUUCCGACCACuACcGUA--GCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 40471 | 0.66 | 0.63847 |
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Target: 5'- -gGCGAAGGCcGGUGA-GGUuuuUCaCCc -3' miRNA: 3'- aaCGCUUCCGaCCACUaCCGu--AGcGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 43266 | 0.67 | 0.592917 |
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Target: 5'- gUGCGccuugccuucgucGAGGCUGGcucgGAuUGGCGcUCGCUc -3' miRNA: 3'- aACGC-------------UUCCGACCa---CU-ACCGU-AGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 44904 | 0.74 | 0.242005 |
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Target: 5'- -cGCGggGGCUGGauugcucGAUGGCAagcugCGCg -3' miRNA: 3'- aaCGCuuCCGACCa------CUACCGUa----GCGg -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 48829 | 0.67 | 0.582972 |
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Target: 5'- --aUGggGGCgacgaGGUGAUGuccuuCAUCGCCa -3' miRNA: 3'- aacGCuuCCGa----CCACUACc----GUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 49706 | 0.69 | 0.476024 |
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Target: 5'- gUUGCGAucGUUGGcgccugccagaaUGAcGGUAUCGCCa -3' miRNA: 3'- -AACGCUucCGACC------------ACUaCCGUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 54197 | 0.71 | 0.353494 |
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Target: 5'- -gGCGccaacuuuucGAGGUUGGUGAUGcGCuUCGUCa -3' miRNA: 3'- aaCGC----------UUCCGACCACUAC-CGuAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 56637 | 0.68 | 0.539264 |
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Target: 5'- -gGCGgcGGCaugGGUcGcgGGCAacuUCGCCc -3' miRNA: 3'- aaCGCuuCCGa--CCA-CuaCCGU---AGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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