Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12333 | 5' | -55.7 | NC_003324.1 | + | 24377 | 0.67 | 0.601781 |
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Target: 5'- cUGCGAuacGGGCUGGccgagaUGGUcgGGCAUgcggauaugcacugCGCCg -3' miRNA: 3'- aACGCU---UCCGACC------ACUA--CCGUA--------------GCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 20582 | 0.67 | 0.616219 |
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Target: 5'- -gGCGAgcGGGauccGGUGcuGUGGCgccGUCGCCa -3' miRNA: 3'- aaCGCU--UCCga--CCAC--UACCG---UAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 30340 | 0.66 | 0.624004 |
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Target: 5'- cUGCGAu-GCUGcauccuccgucagaGccuUGAUGGCGUCGCCc -3' miRNA: 3'- aACGCUucCGAC--------------C---ACUACCGUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 15451 | 0.66 | 0.627342 |
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Target: 5'- -cGCGAAGGUUGccaggGGCGagGCCa -3' miRNA: 3'- aaCGCUUCCGACcacuaCCGUagCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 40471 | 0.66 | 0.63847 |
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Target: 5'- -gGCGAAGGCcGGUGA-GGUuuuUCaCCc -3' miRNA: 3'- aaCGCUUCCGaCCACUaCCGu--AGcGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 30325 | 0.66 | 0.649591 |
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Target: 5'- -cGcCGAuGGUgucGGcGGUGGCGUCGCUc -3' miRNA: 3'- aaC-GCUuCCGa--CCaCUACCGUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 10821 | 0.66 | 0.657367 |
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Target: 5'- -gGUGAugacaacGGcGCUGGUGGUGGCugcacgagugaCGCCc -3' miRNA: 3'- aaCGCU-------UC-CGACCACUACCGua---------GCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 233 | 0.66 | 0.660697 |
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Target: 5'- --uCGAcuuuaGGGCUGGcGGccGCAUCGCCg -3' miRNA: 3'- aacGCU-----UCCGACCaCUacCGUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 26811 | 0.66 | 0.682815 |
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Target: 5'- gUGCGcuuGGCUGGcuuUGGCGcCGCUc -3' miRNA: 3'- aACGCuu-CCGACCacuACCGUaGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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