Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12333 | 5' | -55.7 | NC_003324.1 | + | 56637 | 0.68 | 0.539264 |
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Target: 5'- -gGCGgcGGCaugGGUcGcgGGCAacuUCGCCc -3' miRNA: 3'- aaCGCuuCCGa--CCA-CuaCCGU---AGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 39655 | 0.68 | 0.550099 |
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Target: 5'- --cCGGAGGCUGGUGcUGaCGUggCGCCa -3' miRNA: 3'- aacGCUUCCGACCACuACcGUA--GCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 22486 | 0.68 | 0.561001 |
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Target: 5'- -gGCGAAGGUguucggcgaggUGGUGAcggauUGGgagcuGUCGCCg -3' miRNA: 3'- aaCGCUUCCG-----------ACCACU-----ACCg----UAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 32541 | 0.67 | 0.582972 |
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Target: 5'- -gGCGAAGGCgacgaGG-GcAUGGCugccGUUGCCa -3' miRNA: 3'- aaCGCUUCCGa----CCaC-UACCG----UAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 43266 | 0.67 | 0.592917 |
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Target: 5'- gUGCGccuugccuucgucGAGGCUGGcucgGAuUGGCGcUCGCUc -3' miRNA: 3'- aACGC-------------UUCCGACCa---CU-ACCGU-AGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 40471 | 0.66 | 0.63847 |
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Target: 5'- -gGCGAAGGCcGGUGA-GGUuuuUCaCCc -3' miRNA: 3'- aaCGCUUCCGaCCACUaCCGu--AGcGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 30325 | 0.66 | 0.649591 |
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Target: 5'- -cGcCGAuGGUgucGGcGGUGGCGUCGCUc -3' miRNA: 3'- aaC-GCUuCCGa--CCaCUACCGUAGCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 10821 | 0.66 | 0.657367 |
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Target: 5'- -gGUGAugacaacGGcGCUGGUGGUGGCugcacgagugaCGCCc -3' miRNA: 3'- aaCGCU-------UC-CGACCACUACCGua---------GCGG- -5' |
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| 12333 | 5' | -55.7 | NC_003324.1 | + | 16077 | 1.1 | 0.000634 |
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Target: 5'- uUUGCGAAGGCUGGUGAUGGCAUCGCCg -3' miRNA: 3'- -AACGCUUCCGACCACUACCGUAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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