Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12336 | 5' | -54.3 | NC_003324.1 | + | 51661 | 0.67 | 0.721312 |
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Target: 5'- uUGCUCGGGAAUaCCGAAGGCgccuuacccuUGCGa -3' miRNA: 3'- cGCGAGUUCUUGcGGCUUUCG----------GCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 51765 | 0.7 | 0.534306 |
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Target: 5'- gGCGCUCGAcgcGAACGUCGGucacgaucaggguAAGgCGCGa -3' miRNA: 3'- -CGCGAGUU---CUUGCGGCU-------------UUCgGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 53393 | 0.78 | 0.195019 |
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Target: 5'- uGCGCUCAAcGGCGaCCGAuaguucuuucucAAGCCGCGa -3' miRNA: 3'- -CGCGAGUUcUUGC-GGCU------------UUCGGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 53732 | 0.71 | 0.493232 |
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Target: 5'- aGCGCguaCAGGAA--CCGGAGGCCGCu- -3' miRNA: 3'- -CGCGa--GUUCUUgcGGCUUUCGGCGca -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 54967 | 0.69 | 0.583096 |
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Target: 5'- uCGCUUgcGGGCGCCGAcauucgcgagcauccGGGCCuGCGg -3' miRNA: 3'- cGCGAGuuCUUGCGGCU---------------UUCGG-CGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 55330 | 0.67 | 0.742521 |
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Target: 5'- aGCgGCUCAAGGAuCGCUGGAa--CGCGa -3' miRNA: 3'- -CG-CGAGUUCUU-GCGGCUUucgGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 57230 | 0.66 | 0.752962 |
|
Target: 5'- gGCGCaCAAGAAcuuCGCCaAGGGUCGCa- -3' miRNA: 3'- -CGCGaGUUCUU---GCGGcUUUCGGCGca -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 57276 | 0.72 | 0.452675 |
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Target: 5'- uGCGCUCAGaugcucAACGCCGGucguGCCGCc- -3' miRNA: 3'- -CGCGAGUUc-----UUGCGGCUuu--CGGCGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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