Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12336 | 5' | -54.3 | NC_003324.1 | + | 26255 | 0.67 | 0.699747 |
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Target: 5'- cCGCagCGAGAcCGCCGAcagaaugaugcAGGCCGCc- -3' miRNA: 3'- cGCGa-GUUCUuGCGGCU-----------UUCGGCGca -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 18906 | 0.67 | 0.688858 |
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Target: 5'- gGCGUgaUgGAGAcugGCGCCGAccuucGCCGCGc -3' miRNA: 3'- -CGCG--AgUUCU---UGCGGCUuu---CGGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 31128 | 0.67 | 0.688858 |
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Target: 5'- aGCGCgucagCAGGAguugaACGCCGA---CCGCGc -3' miRNA: 3'- -CGCGa----GUUCU-----UGCGGCUuucGGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 50626 | 0.67 | 0.688858 |
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Target: 5'- uGCGCUCGgcgucgagcAGGucaACGCgGGugcuGGCUGCGUg -3' miRNA: 3'- -CGCGAGU---------UCU---UGCGgCUu---UCGGCGCA- -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 26203 | 0.68 | 0.666929 |
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Target: 5'- aCGgUCGAGAucagguaGCCGAAGGCC-CGUu -3' miRNA: 3'- cGCgAGUUCUug-----CGGCUUUCGGcGCA- -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 21220 | 0.68 | 0.666929 |
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Target: 5'- uCGCUCAcuGACgagGCCGAGAuGCUGCGc -3' miRNA: 3'- cGCGAGUucUUG---CGGCUUU-CGGCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 42015 | 0.68 | 0.633813 |
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Target: 5'- aGCGCaUCGGGAACGCgguaccucCGGAuGCgGCGa -3' miRNA: 3'- -CGCG-AGUUCUUGCG--------GCUUuCGgCGCa -5' |
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| 12336 | 5' | -54.3 | NC_003324.1 | + | 14468 | 0.66 | 0.793369 |
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Target: 5'- cGCGCUCGguugcGGcGCGuuGAuuggcagcAGCCGUGg -3' miRNA: 3'- -CGCGAGU-----UCuUGCggCUu-------UCGGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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