Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12345 | 3' | -53 | NC_003324.1 | + | 24504 | 0.66 | 0.850064 |
Target: 5'- nCAAUGUUCGc-AUCGCC-CGaCGCGCa -3' miRNA: 3'- -GUUGCAGGUuuUGGCGGaGCaGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 44670 | 0.66 | 0.841405 |
Target: 5'- gCAACGggcaguUCCuc-GCCGCCgUGUCGCuGCc -3' miRNA: 3'- -GUUGC------AGGuuuUGGCGGaGCAGCG-CG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 5816 | 0.66 | 0.841405 |
Target: 5'- aAACGguagCCGGuGCCGCCaacggagguuUCGgUUGCGCc -3' miRNA: 3'- gUUGCa---GGUUuUGGCGG----------AGC-AGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 53085 | 0.66 | 0.874625 |
Target: 5'- uCAGCGuaaUCCuucuGGAuCCGCUUCauuggcggcagGUCGCGCu -3' miRNA: 3'- -GUUGC---AGGu---UUU-GGCGGAG-----------CAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 19043 | 0.66 | 0.872268 |
Target: 5'- -cGCGUCauucGAGCCuuugcagcccauuuGCCcuuugUCGUCGCGCu -3' miRNA: 3'- guUGCAGgu--UUUGG--------------CGG-----AGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 20846 | 0.66 | 0.866682 |
Target: 5'- ---gGUCCAugucGGCCGCaucCUCGaCGUGCa -3' miRNA: 3'- guugCAGGUu---UUGGCG---GAGCaGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 49883 | 0.66 | 0.866682 |
Target: 5'- cCGGCGggcaAAGAUCGCUgCGUCgGCGCg -3' miRNA: 3'- -GUUGCagg-UUUUGGCGGaGCAG-CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 39682 | 0.66 | 0.858492 |
Target: 5'- aCAuCGUgCuuGAugUGCCgcaCGUCGCGCc -3' miRNA: 3'- -GUuGCAgGu-UUugGCGGa--GCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 20676 | 0.66 | 0.850064 |
Target: 5'- gCGAUGcUUgGAAGCCGUCUCuucgagcuccGUUGCGCc -3' miRNA: 3'- -GUUGC-AGgUUUUGGCGGAG----------CAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 19374 | 0.66 | 0.832526 |
Target: 5'- uCAGCGauggaUCGAcGCCGgCgcgCGUCGCGCc -3' miRNA: 3'- -GUUGCa----GGUUuUGGCgGa--GCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 33418 | 0.66 | 0.850064 |
Target: 5'- gAACG-CCcGAGCCGCUUCaUCgGCGUu -3' miRNA: 3'- gUUGCaGGuUUUGGCGGAGcAG-CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 13983 | 0.67 | 0.794981 |
Target: 5'- aCAACG-CUAuAACgGgC-CGUCGCGCa -3' miRNA: 3'- -GUUGCaGGUuUUGgCgGaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31336 | 0.67 | 0.804651 |
Target: 5'- aAGCGUugcaucgaucCCAGGAugcCCGCCUCGUCaucUGCa -3' miRNA: 3'- gUUGCA----------GGUUUU---GGCGGAGCAGc--GCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 24925 | 0.67 | 0.823433 |
Target: 5'- gCAGauugCCAAGacgGCCGaCCUUGUCgGCGCg -3' miRNA: 3'- -GUUgca-GGUUU---UGGC-GGAGCAG-CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 16304 | 0.67 | 0.823433 |
Target: 5'- -cGCGUCggCAcgGCCuGCCcCGUCGCGg -3' miRNA: 3'- guUGCAG--GUuuUGG-CGGaGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 53717 | 0.67 | 0.823433 |
Target: 5'- aCGGCGguaguUCUuuuGGAGCCGgCgacguUCGUCGCGCa -3' miRNA: 3'- -GUUGC-----AGG---UUUUGGCgG-----AGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 24529 | 0.67 | 0.823433 |
Target: 5'- gAAUGUCCAGGACgUGCag-GUCgGCGCa -3' miRNA: 3'- gUUGCAGGUUUUG-GCGgagCAG-CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 41330 | 0.67 | 0.814138 |
Target: 5'- aCGAUGUC--GAACgGCCUCGaaaUCgGCGCu -3' miRNA: 3'- -GUUGCAGguUUUGgCGGAGC---AG-CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 29460 | 0.67 | 0.823433 |
Target: 5'- uCGACGaugaCAcgGCCGCCUggcugcgggcgCGUCGgGCg -3' miRNA: 3'- -GUUGCag--GUuuUGGCGGA-----------GCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 41291 | 0.67 | 0.804651 |
Target: 5'- uCGACGUCCAucAAGCCgugcgGCCUUuccaUCGCGa -3' miRNA: 3'- -GUUGCAGGU--UUUGG-----CGGAGc---AGCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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