Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12345 | 3' | -53 | NC_003324.1 | + | 1413 | 0.73 | 0.495148 |
Target: 5'- gGGCGUUCu---UCGCCUCGUCGaGCa -3' miRNA: 3'- gUUGCAGGuuuuGGCGGAGCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 1761 | 0.75 | 0.370334 |
Target: 5'- uCAGCGUgCCAggacGAACCGCCgucagCGUCagcagGCGCa -3' miRNA: 3'- -GUUGCA-GGU----UUUGGCGGa----GCAG-----CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 2627 | 0.68 | 0.754702 |
Target: 5'- aCGGCGUgCCGucuGCCGUC-CGUCGCc- -3' miRNA: 3'- -GUUGCA-GGUuu-UGGCGGaGCAGCGcg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 4786 | 0.7 | 0.667738 |
Target: 5'- gGAUuUCCAGccAGCCGCCcuggucgUUGUUGCGCa -3' miRNA: 3'- gUUGcAGGUU--UUGGCGG-------AGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 5590 | 0.77 | 0.290054 |
Target: 5'- gAGCGaUCCuuAcCCGCCuUCGUCGUGCg -3' miRNA: 3'- gUUGC-AGGuuUuGGCGG-AGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 5816 | 0.66 | 0.841405 |
Target: 5'- aAACGguagCCGGuGCCGCCaacggagguuUCGgUUGCGCc -3' miRNA: 3'- gUUGCa---GGUUuUGGCGG----------AGC-AGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 5915 | 0.67 | 0.785141 |
Target: 5'- --gUGUCCGGAACCGCC-CGguaGUGa -3' miRNA: 3'- guuGCAGGUUUUGGCGGaGCag-CGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 7750 | 0.69 | 0.701606 |
Target: 5'- -uAUGUCCgAGAGCaCGCCUCGcagggagGCGCg -3' miRNA: 3'- guUGCAGG-UUUUG-GCGGAGCag-----CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 7796 | 0.67 | 0.804651 |
Target: 5'- -uGCGgCCGu-GCUGaCCUCGuUCGCGCc -3' miRNA: 3'- guUGCaGGUuuUGGC-GGAGC-AGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 8276 | 0.69 | 0.690736 |
Target: 5'- aCAACGUCCGuAGUCGUCgcCGUCGCaGCc -3' miRNA: 3'- -GUUGCAGGUuUUGGCGGa-GCAGCG-CG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 11716 | 0.75 | 0.38811 |
Target: 5'- uCGGCGUCa--GACCGCCgUCGUUcaaGCGCa -3' miRNA: 3'- -GUUGCAGguuUUGGCGG-AGCAG---CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 12404 | 0.68 | 0.77514 |
Target: 5'- aCAAUGU-CGGAGCCGa--CGUUGCGCg -3' miRNA: 3'- -GUUGCAgGUUUUGGCggaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 12912 | 0.7 | 0.635755 |
Target: 5'- aCAGCGUCUgcGGAGCC-CCUUGcggCGCGUc -3' miRNA: 3'- -GUUGCAGG--UUUUGGcGGAGCa--GCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 13983 | 0.67 | 0.794981 |
Target: 5'- aCAACG-CUAuAACgGgC-CGUCGCGCa -3' miRNA: 3'- -GUUGCaGGUuUUGgCgGaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 14454 | 0.69 | 0.723129 |
Target: 5'- cCGGCGUCCAAgcaucggcgGACCuuGCUggcacCGUCGCGg -3' miRNA: 3'- -GUUGCAGGUU---------UUGG--CGGa----GCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 14607 | 0.7 | 0.65783 |
Target: 5'- gAGCG-CCAG---CGCCUUGUCGCGg -3' miRNA: 3'- gUUGCaGGUUuugGCGGAGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 15983 | 0.74 | 0.43492 |
Target: 5'- --uCGUUUu--GCCGCUUCGUCGCGUc -3' miRNA: 3'- guuGCAGGuuuUGGCGGAGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 16034 | 0.68 | 0.77514 |
Target: 5'- cCAACGcCC---ACCGCCUUGUCGa-- -3' miRNA: 3'- -GUUGCaGGuuuUGGCGGAGCAGCgcg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 16304 | 0.67 | 0.823433 |
Target: 5'- -cGCGUCggCAcgGCCuGCCcCGUCGCGg -3' miRNA: 3'- guUGCAG--GUuuUGG-CGGaGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 17108 | 0.7 | 0.646799 |
Target: 5'- uGAUGUCCucGAUCGCCUCa-CGgGCg -3' miRNA: 3'- gUUGCAGGuuUUGGCGGAGcaGCgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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