Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12345 | 3' | -53 | NC_003324.1 | + | 21638 | 1.12 | 0.001323 |
Target: 5'- cCAACGUCCAAAACCGCCUCGUCGCGCu -3' miRNA: 3'- -GUUGCAGGUUUUGGCGGAGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 28624 | 0.84 | 0.106635 |
Target: 5'- uCGugGcCCGGAucguugcggGCCGCCUUGUCGCGCg -3' miRNA: 3'- -GUugCaGGUUU---------UGGCGGAGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 19527 | 0.79 | 0.218227 |
Target: 5'- aGGCGUCCAucAGCCGCUUCa-CGCGCa -3' miRNA: 3'- gUUGCAGGUu-UUGGCGGAGcaGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 5590 | 0.77 | 0.290054 |
Target: 5'- gAGCGaUCCuuAcCCGCCuUCGUCGUGCg -3' miRNA: 3'- gUUGC-AGGuuUuGGCGG-AGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 1761 | 0.75 | 0.370334 |
Target: 5'- uCAGCGUgCCAggacGAACCGCCgucagCGUCagcagGCGCa -3' miRNA: 3'- -GUUGCA-GGU----UUUGGCGGa----GCAG-----CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 11716 | 0.75 | 0.38811 |
Target: 5'- uCGGCGUCa--GACCGCCgUCGUUcaaGCGCa -3' miRNA: 3'- -GUUGCAGguuUUGGCGG-AGCAG---CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31435 | 0.74 | 0.397205 |
Target: 5'- --cCGUCCGGcAACUGCCaUGUCGUGCu -3' miRNA: 3'- guuGCAGGUU-UUGGCGGaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 55405 | 0.74 | 0.406436 |
Target: 5'- uCAGCG-CCGAAGcCCGCCUugaacaguggCGUCGUGUg -3' miRNA: 3'- -GUUGCaGGUUUU-GGCGGA----------GCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 28882 | 0.74 | 0.415801 |
Target: 5'- uCAACGUCCcaccacuGGCCgGCCUCGUCcuuCGCu -3' miRNA: 3'- -GUUGCAGGuu-----UUGG-CGGAGCAGc--GCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 15983 | 0.74 | 0.43492 |
Target: 5'- --uCGUUUu--GCCGCUUCGUCGCGUc -3' miRNA: 3'- guuGCAGGuuuUGGCGGAGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 30256 | 0.73 | 0.454539 |
Target: 5'- gGGCGUCUucgaAGAAUUGCCgcagggCGUUGCGCa -3' miRNA: 3'- gUUGCAGG----UUUUGGCGGa-----GCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 1413 | 0.73 | 0.495148 |
Target: 5'- gGGCGUUCu---UCGCCUCGUCGaGCa -3' miRNA: 3'- gUUGCAGGuuuuGGCGGAGCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31513 | 0.72 | 0.516064 |
Target: 5'- aAugGUCgCAGuccGCCGuCCUCGUCGUGa -3' miRNA: 3'- gUugCAG-GUUu--UGGC-GGAGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 23736 | 0.72 | 0.537326 |
Target: 5'- -cACGUCacgaAGAGCUGCgaCGUUGCGCc -3' miRNA: 3'- guUGCAGg---UUUUGGCGgaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 47634 | 0.71 | 0.569754 |
Target: 5'- aCGGCGaCC-GGGCCGCCaUCGUCGaGCu -3' miRNA: 3'- -GUUGCaGGuUUUGGCGG-AGCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 20749 | 0.71 | 0.580676 |
Target: 5'- aCGGCuUCCAAGcaucGCCGCCUgGUCGaaGCa -3' miRNA: 3'- -GUUGcAGGUUU----UGGCGGAgCAGCg-CG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 29793 | 0.71 | 0.602641 |
Target: 5'- uCGGCGUCCAca--CGCacaGUCGCGCc -3' miRNA: 3'- -GUUGCAGGUuuugGCGgagCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 12912 | 0.7 | 0.635755 |
Target: 5'- aCAGCGUCUgcGGAGCC-CCUUGcggCGCGUc -3' miRNA: 3'- -GUUGCAGG--UUUUGGcGGAGCa--GCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 49579 | 0.7 | 0.635755 |
Target: 5'- ---aGUgCCAGAauGCCGcCCUCGUUGCGg -3' miRNA: 3'- guugCA-GGUUU--UGGC-GGAGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 17261 | 0.7 | 0.645696 |
Target: 5'- -cGCGUCCGAcaGACgcgccagCGCCUCGUCaauCGCu -3' miRNA: 3'- guUGCAGGUU--UUG-------GCGGAGCAGc--GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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