Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12345 | 3' | -53 | NC_003324.1 | + | 41291 | 0.67 | 0.804651 |
Target: 5'- uCGACGUCCAucAAGCCgugcgGCCUUuccaUCGCGa -3' miRNA: 3'- -GUUGCAGGU--UUUGG-----CGGAGc---AGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 39018 | 0.7 | 0.668837 |
Target: 5'- uGACGaUCUucu-CCGCCuUCGUCGgGCu -3' miRNA: 3'- gUUGC-AGGuuuuGGCGG-AGCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 8276 | 0.69 | 0.690736 |
Target: 5'- aCAACGUCCGuAGUCGUCgcCGUCGCaGCc -3' miRNA: 3'- -GUUGCAGGUuUUGGCGGa-GCAGCG-CG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 7750 | 0.69 | 0.701606 |
Target: 5'- -uAUGUCCgAGAGCaCGCCUCGcagggagGCGCg -3' miRNA: 3'- guUGCAGG-UUUUG-GCGGAGCag-----CGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 14454 | 0.69 | 0.723129 |
Target: 5'- cCGGCGUCCAAgcaucggcgGACCuuGCUggcacCGUCGCGg -3' miRNA: 3'- -GUUGCAGGUU---------UUGG--CGGa----GCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 34093 | 0.68 | 0.754702 |
Target: 5'- --cUGUCCAugAGACCGagUCGaacUCGCGCa -3' miRNA: 3'- guuGCAGGU--UUUGGCggAGC---AGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 21299 | 0.68 | 0.754702 |
Target: 5'- -cGCGUUC---GCCGCCUCGcCG-GCg -3' miRNA: 3'- guUGCAGGuuuUGGCGGAGCaGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 13983 | 0.67 | 0.794981 |
Target: 5'- aCAACG-CUAuAACgGgC-CGUCGCGCa -3' miRNA: 3'- -GUUGCaGGUuUUGgCgGaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31336 | 0.67 | 0.804651 |
Target: 5'- aAGCGUugcaucgaucCCAGGAugcCCGCCUCGUCaucUGCa -3' miRNA: 3'- gUUGCA----------GGUUUU---GGCGGAGCAGc--GCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31237 | 0.7 | 0.65783 |
Target: 5'- cCAGCG-CCAGcGCCGCCggcCGcacUGCGCg -3' miRNA: 3'- -GUUGCaGGUUuUGGCGGa--GCa--GCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 17108 | 0.7 | 0.646799 |
Target: 5'- uGAUGUCCucGAUCGCCUCa-CGgGCg -3' miRNA: 3'- gUUGCAGGuuUUGGCGGAGcaGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 29793 | 0.71 | 0.602641 |
Target: 5'- uCGGCGUCCAca--CGCacaGUCGCGCc -3' miRNA: 3'- -GUUGCAGGUuuugGCGgagCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 19527 | 0.79 | 0.218227 |
Target: 5'- aGGCGUCCAucAGCCGCUUCa-CGCGCa -3' miRNA: 3'- gUUGCAGGUu-UUGGCGGAGcaGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31435 | 0.74 | 0.397205 |
Target: 5'- --cCGUCCGGcAACUGCCaUGUCGUGCu -3' miRNA: 3'- guuGCAGGUU-UUGGCGGaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 55405 | 0.74 | 0.406436 |
Target: 5'- uCAGCG-CCGAAGcCCGCCUugaacaguggCGUCGUGUg -3' miRNA: 3'- -GUUGCaGGUUUU-GGCGGA----------GCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 28882 | 0.74 | 0.415801 |
Target: 5'- uCAACGUCCcaccacuGGCCgGCCUCGUCcuuCGCu -3' miRNA: 3'- -GUUGCAGGuu-----UUGG-CGGAGCAGc--GCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 1413 | 0.73 | 0.495148 |
Target: 5'- gGGCGUUCu---UCGCCUCGUCGaGCa -3' miRNA: 3'- gUUGCAGGuuuuGGCGGAGCAGCgCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 31513 | 0.72 | 0.516064 |
Target: 5'- aAugGUCgCAGuccGCCGuCCUCGUCGUGa -3' miRNA: 3'- gUugCAG-GUUu--UGGC-GGAGCAGCGCg -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 23736 | 0.72 | 0.537326 |
Target: 5'- -cACGUCacgaAGAGCUGCgaCGUUGCGCc -3' miRNA: 3'- guUGCAGg---UUUUGGCGgaGCAGCGCG- -5' |
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12345 | 3' | -53 | NC_003324.1 | + | 47634 | 0.71 | 0.569754 |
Target: 5'- aCGGCGaCC-GGGCCGCCaUCGUCGaGCu -3' miRNA: 3'- -GUUGCaGGuUUUGGCGG-AGCAGCgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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