Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12349 | 5' | -55.9 | NC_003324.1 | + | 25491 | 0.71 | 0.354781 |
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Target: 5'- cGCCUUCUA--CCCGaggaCGGUAGCGUCGc -3' miRNA: 3'- uCGGAAGAUaaGGGC----GCCGUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 7875 | 0.72 | 0.329975 |
|
Target: 5'- aAGCCU--UGUUCgCCGCGGCGGCaaaGCCc -3' miRNA: 3'- -UCGGAagAUAAG-GGCGCCGUUG---CGGu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 3179 | 0.72 | 0.321205 |
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Target: 5'- cGCUUUCUGUUCgCCucgaauggcaagaGCGGgAGCGCCGu -3' miRNA: 3'- uCGGAAGAUAAG-GG-------------CGCCgUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 46012 | 0.73 | 0.287156 |
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Target: 5'- cAGCCUgcggcgucgcuugcuUCgcUUCCgaaGCGGCGGCGCCGa -3' miRNA: 3'- -UCGGA---------------AGauAAGGg--CGCCGUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 11573 | 0.75 | 0.213701 |
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Target: 5'- cAGCCgaugCUGcUCCCGCGGCuccUGCCGg -3' miRNA: 3'- -UCGGaa--GAUaAGGGCGCCGuu-GCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 23676 | 1.09 | 0.000852 |
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Target: 5'- aAGCCUUCUAUUCCCGCGGCAACGCCAa -3' miRNA: 3'- -UCGGAAGAUAAGGGCGCCGUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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