Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12349 | 5' | -55.9 | NC_003324.1 | + | 33766 | 0.67 | 0.593601 |
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Target: 5'- aAGCCUgugAUgCCUugGCGGCAGCGCa- -3' miRNA: 3'- -UCGGAagaUAaGGG--CGCCGUUGCGgu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 42227 | 0.66 | 0.63659 |
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Target: 5'- cGGCCcauggUCUGUUgcugaucCCCGCGGCGGCa--- -3' miRNA: 3'- -UCGGa----AGAUAA-------GGGCGCCGUUGcggu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 39271 | 0.66 | 0.648728 |
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Target: 5'- cGCCUaugaUCgGUUgCCGCcguGGCuGCGCCAg -3' miRNA: 3'- uCGGA----AGaUAAgGGCG---CCGuUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 51009 | 0.66 | 0.670742 |
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Target: 5'- aGGCCUcaUAUUCuCCGgGGCGcuCGUCAa -3' miRNA: 3'- -UCGGAagAUAAG-GGCgCCGUu-GCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 18110 | 0.66 | 0.681699 |
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Target: 5'- cGCCUaUCgcauggCCCGUGGCGcCgGCCAa -3' miRNA: 3'- uCGGA-AGauaa--GGGCGCCGUuG-CGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 23676 | 1.09 | 0.000852 |
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Target: 5'- aAGCCUUCUAUUCCCGCGGCAACGCCAa -3' miRNA: 3'- -UCGGAAGAUAAGGGCGCCGUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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