Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12349 | 5' | -55.9 | NC_003324.1 | + | 46012 | 0.73 | 0.287156 |
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Target: 5'- cAGCCUgcggcgucgcuugcuUCgcUUCCgaaGCGGCGGCGCCGa -3' miRNA: 3'- -UCGGA---------------AGauAAGGg--CGCCGUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 47938 | 0.69 | 0.456404 |
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Target: 5'- cGCCggUCagguacgGUUUCaGCGGCAGCGCCGc -3' miRNA: 3'- uCGGa-AGa------UAAGGgCGCCGUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 49028 | 0.68 | 0.550026 |
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Target: 5'- cGGCCaugcUUCUcaccucUCCCGCGGCGGCGg-- -3' miRNA: 3'- -UCGG----AAGAua----AGGGCGCCGUUGCggu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 51009 | 0.66 | 0.670742 |
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Target: 5'- aGGCCUcaUAUUCuCCGgGGCGcuCGUCAa -3' miRNA: 3'- -UCGGAagAUAAG-GGCgCCGUu-GCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 51538 | 0.68 | 0.528598 |
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Target: 5'- uGGCUUgggCUGccgUCuuGCGGCAACGgCGu -3' miRNA: 3'- -UCGGAa--GAUa--AGggCGCCGUUGCgGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 51584 | 0.69 | 0.466405 |
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Target: 5'- cGCCUUCggUAUUCCCGa-GCAAgaGCCAc -3' miRNA: 3'- uCGGAAG--AUAAGGGCgcCGUUg-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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