Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12349 | 5' | -55.9 | NC_003324.1 | + | 40832 | 0.66 | 0.648728 |
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Target: 5'- gAGUUUUCUAUUUCCGCcucGCAGCGgUg -3' miRNA: 3'- -UCGGAAGAUAAGGGCGc--CGUUGCgGu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 38081 | 0.66 | 0.659748 |
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Target: 5'- cGCCUUCUGgcgaagCGCGuCGGCGCCGu -3' miRNA: 3'- uCGGAAGAUaagg--GCGCcGUUGCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 23408 | 0.66 | 0.659748 |
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Target: 5'- cGGCUUUCUcgUUggCCGCgucGGUGGCGCCc -3' miRNA: 3'- -UCGGAAGAuaAG--GGCG---CCGUUGCGGu -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 51009 | 0.66 | 0.670742 |
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Target: 5'- aGGCCUcaUAUUCuCCGgGGCGcuCGUCAa -3' miRNA: 3'- -UCGGAagAUAAG-GGCgCCGUu-GCGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 18110 | 0.66 | 0.681699 |
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Target: 5'- cGCCUaUCgcauggCCCGUGGCGcCgGCCAa -3' miRNA: 3'- uCGGA-AGauaa--GGGCGCCGUuG-CGGU- -5' |
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| 12349 | 5' | -55.9 | NC_003324.1 | + | 22599 | 0.66 | 0.681699 |
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Target: 5'- cGGCCUUUUGgcugCCUGCcGCGuUGCCGa -3' miRNA: 3'- -UCGGAAGAUaa--GGGCGcCGUuGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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