Results 1 - 20 of 75 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12355 | 5' | -52.8 | NC_003324.1 | + | 38119 | 0.66 | 0.872514 |
Target: 5'- cGCCGucucguGUCGgUCGgCGGAauguuGACGCGCc -3' miRNA: 3'- aCGGCu-----UAGCaGGUgGUCU-----UUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 24562 | 0.66 | 0.872514 |
Target: 5'- gUGCCGGAagaUCGU-CACCGcu-GCGgGCg -3' miRNA: 3'- -ACGGCUU---AGCAgGUGGUcuuUGCgCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 38001 | 0.66 | 0.872514 |
Target: 5'- aUGCCGGAgCGguugCgGCgCAcGggGCGCGUc -3' miRNA: 3'- -ACGGCUUaGCa---GgUG-GU-CuuUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 12603 | 0.66 | 0.872514 |
Target: 5'- cGUCGccUCGaacugCCACCAGcccgucguuGGACGCGUa -3' miRNA: 3'- aCGGCuuAGCa----GGUGGUC---------UUUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 2755 | 0.66 | 0.872514 |
Target: 5'- uUGCCGAAgCGgCCACCgcgcuGGAAGgGCa- -3' miRNA: 3'- -ACGGCUUaGCaGGUGG-----UCUUUgCGcg -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 34438 | 0.66 | 0.870123 |
Target: 5'- gGCCGAcggcacauuggcaagCGUCgGCgGGAuuuCGCGCu -3' miRNA: 3'- aCGGCUua-------------GCAGgUGgUCUuu-GCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 32833 | 0.66 | 0.864457 |
Target: 5'- aGCCGccUCGaucaUCGCCAcuGACGCGCc -3' miRNA: 3'- aCGGCuuAGCa---GGUGGUcuUUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 19864 | 0.66 | 0.864457 |
Target: 5'- cUGUCGu-UCGUCCagaaaaugccGCCGGggGaCGCGa -3' miRNA: 3'- -ACGGCuuAGCAGG----------UGGUCuuU-GCGCg -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 47780 | 0.66 | 0.864457 |
Target: 5'- aGCau-GUCGUCgaggagGCCGcGAAGCGCGCc -3' miRNA: 3'- aCGgcuUAGCAGg-----UGGU-CUUUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 27951 | 0.66 | 0.864457 |
Target: 5'- gGCCGGcuaCG-CCAgCGaggacGAAGCGCGCg -3' miRNA: 3'- aCGGCUua-GCaGGUgGU-----CUUUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 8855 | 0.66 | 0.862815 |
Target: 5'- gGCCGucgggagcauuUCGguagCCGCgGGAGAUGCGg -3' miRNA: 3'- aCGGCuu---------AGCa---GGUGgUCUUUGCGCg -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 34701 | 0.66 | 0.856153 |
Target: 5'- cGCCGAGgugCGcaaaCugCAGA-GCGCGUc -3' miRNA: 3'- aCGGCUUa--GCag--GugGUCUuUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 25941 | 0.66 | 0.856153 |
Target: 5'- gGUCGAucgcgAUCGcUCCACC----ACGCGCc -3' miRNA: 3'- aCGGCU-----UAGC-AGGUGGucuuUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 15201 | 0.66 | 0.853614 |
Target: 5'- cGCCGuGUCGa-CGCCgAGAccgauggcauccauGACGCGCu -3' miRNA: 3'- aCGGCuUAGCagGUGG-UCU--------------UUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 22563 | 0.66 | 0.847609 |
Target: 5'- gGCCGAAaUGUUCA-CGGucAUGCGCu -3' miRNA: 3'- aCGGCUUaGCAGGUgGUCuuUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 25829 | 0.66 | 0.847609 |
Target: 5'- cGcCCGGuuUCGUCCucaaGCUGGAugcGGCGCGUg -3' miRNA: 3'- aC-GGCUu-AGCAGG----UGGUCU---UUGCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 6216 | 0.66 | 0.838836 |
Target: 5'- gGCgCGggUCaGUCCGCUAucGGCguagGCGCg -3' miRNA: 3'- aCG-GCuuAG-CAGGUGGUcuUUG----CGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 23469 | 0.66 | 0.838836 |
Target: 5'- aGCCGAGcgCGUCaaagCGGAGugGCGa -3' miRNA: 3'- aCGGCUUa-GCAGgug-GUCUUugCGCg -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 50619 | 0.67 | 0.829841 |
Target: 5'- aGCUGcaGAUCGUCaacgAUCAGAucGAgGCGCa -3' miRNA: 3'- aCGGC--UUAGCAGg---UGGUCU--UUgCGCG- -5' |
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12355 | 5' | -52.8 | NC_003324.1 | + | 45580 | 0.67 | 0.829841 |
Target: 5'- uUGCgGggUUuuaCCugCGGAuguGCGCGCc -3' miRNA: 3'- -ACGgCuuAGca-GGugGUCUu--UGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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