Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12364 | 5' | -62.3 | NC_003324.1 | + | 11842 | 0.66 | 0.393724 |
Target: 5'- uGUCCuGCgCGAcCGGGC-CGGGCaCGGCa -3' miRNA: 3'- -UAGG-CG-GCUaGCUCGcGCUCGgGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 43718 | 0.66 | 0.39286 |
Target: 5'- -aCCGCCugcugccaaucggGAUCaGGGuCGCGAcugugaaagGCCCGGUa -3' miRNA: 3'- uaGGCGG-------------CUAG-CUC-GCGCU---------CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 54609 | 0.66 | 0.388561 |
Target: 5'- --aCGUCGAUCGAGCaagaucgaaagacuCGAggacgugcGCCCGGCu -3' miRNA: 3'- uagGCGGCUAGCUCGc-------------GCU--------CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 32108 | 0.66 | 0.388561 |
Target: 5'- -gUCGCCGGUgaggcCGAGUGCcuuggucuccgccucGGGCCgGGCc -3' miRNA: 3'- uaGGCGGCUA-----GCUCGCG---------------CUCGGgCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 16241 | 0.66 | 0.385144 |
Target: 5'- -cCCGCC--UCGGuGCcaGCGAuGCCUGGCg -3' miRNA: 3'- uaGGCGGcuAGCU-CG--CGCU-CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 48434 | 0.66 | 0.385144 |
Target: 5'- ---aGCCGGcuUgGAGUGCGAGCaCGGUg -3' miRNA: 3'- uaggCGGCU--AgCUCGCGCUCGgGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14072 | 0.66 | 0.384293 |
Target: 5'- gAUCCGCaGAUCGgcaaagcAGCGCucaaggaagcgGAGaCCGGCg -3' miRNA: 3'- -UAGGCGgCUAGC-------UCGCG-----------CUCgGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 40516 | 0.66 | 0.376689 |
Target: 5'- -cCCGauggagccaCGAUCGAGCGCGgcgagaAGCCUcuGGUg -3' miRNA: 3'- uaGGCg--------GCUAGCUCGCGC------UCGGG--CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 45974 | 0.66 | 0.368361 |
Target: 5'- --gCGCCGaAUCGAGgaGCGuGCCCGa- -3' miRNA: 3'- uagGCGGC-UAGCUCg-CGCuCGGGCcg -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 48184 | 0.66 | 0.368361 |
Target: 5'- aGUCCGCU--UCGAGCaGC-AGCCacguCGGCg -3' miRNA: 3'- -UAGGCGGcuAGCUCG-CGcUCGG----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 43687 | 0.66 | 0.360159 |
Target: 5'- uGUCCGagCaGUCGAGCGCGAccGCCaaguaucaGGCc -3' miRNA: 3'- -UAGGCg-GcUAGCUCGCGCU--CGGg-------CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 29666 | 0.66 | 0.352086 |
Target: 5'- uUCUGcCCGAggaaUCGAGCGaCGAGgCCUugaGGCc -3' miRNA: 3'- uAGGC-GGCU----AGCUCGC-GCUC-GGG---CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 26133 | 0.66 | 0.352086 |
Target: 5'- ---aGCCGcgcUUGAGCuaGAGCgCCGGCa -3' miRNA: 3'- uaggCGGCu--AGCUCGcgCUCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 16522 | 0.67 | 0.344142 |
Target: 5'- ---aGCgGAUCGGccuGUGCGuGCUCGGCg -3' miRNA: 3'- uaggCGgCUAGCU---CGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 18272 | 0.67 | 0.344142 |
Target: 5'- -aCCGCUGAcggaaUCGGccgacGCGCGGGCaaucgcugccCCGGCc -3' miRNA: 3'- uaGGCGGCU-----AGCU-----CGCGCUCG----------GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 21401 | 0.67 | 0.344142 |
Target: 5'- cGUCCGCgcAUCGAGCGUGuuCuuGGUg -3' miRNA: 3'- -UAGGCGgcUAGCUCGCGCucGggCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 53654 | 0.67 | 0.336327 |
Target: 5'- -aCCGCCGugcggcaccuUgGAGCGcCGAGCUugauaCGGCg -3' miRNA: 3'- uaGGCGGCu---------AgCUCGC-GCUCGG-----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 19926 | 0.67 | 0.328643 |
Target: 5'- aAUUCGCCGGUCuGAuaGaCGAGCCCaGUc -3' miRNA: 3'- -UAGGCGGCUAG-CUcgC-GCUCGGGcCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 40330 | 0.67 | 0.328643 |
Target: 5'- -aCCGCaCGcaaggcggcaGUCGAGCGgGAGCCa-GCa -3' miRNA: 3'- uaGGCG-GC----------UAGCUCGCgCUCGGgcCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 24276 | 0.67 | 0.313666 |
Target: 5'- uUCuCGUCGAUCuGGGCacgGCgGAGCuuGGCg -3' miRNA: 3'- uAG-GCGGCUAG-CUCG---CG-CUCGggCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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