Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12364 | 5' | -62.3 | NC_003324.1 | + | 1591 | 0.68 | 0.271862 |
Target: 5'- -gCCGCCgGAUCcu-CGCuGGCCCGGCc -3' miRNA: 3'- uaGGCGG-CUAGcucGCGcUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 2836 | 0.69 | 0.228885 |
Target: 5'- uUUCGCCGggCGAGgGgGAGCgucaaCGGCc -3' miRNA: 3'- uAGGCGGCuaGCUCgCgCUCGg----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 4554 | 0.71 | 0.177574 |
Target: 5'- -gCCGCgccgUGAUCGAGCGCGcgcaGGUCCaGGCc -3' miRNA: 3'- uaGGCG----GCUAGCUCGCGC----UCGGG-CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 5965 | 0.7 | 0.21176 |
Target: 5'- -cCCGCCGAaUGgcuaugcAGCGCGAGCaCGGUg -3' miRNA: 3'- uaGGCGGCUaGC-------UCGCGCUCGgGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 8192 | 0.68 | 0.285278 |
Target: 5'- -aCUGCCcggCG-GCGCGcGCCUGGCa -3' miRNA: 3'- uaGGCGGcuaGCuCGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 8220 | 0.76 | 0.077669 |
Target: 5'- -aUCGCa-GUCGAGCGCGcagcuGCCCGGCg -3' miRNA: 3'- uaGGCGgcUAGCUCGCGCu----CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 10722 | 0.68 | 0.265346 |
Target: 5'- gGUCuCGCCaGUCGuAGCGacacgGAGCCaCGGCc -3' miRNA: 3'- -UAG-GCGGcUAGC-UCGCg----CUCGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 11842 | 0.66 | 0.393724 |
Target: 5'- uGUCCuGCgCGAcCGGGC-CGGGCaCGGCa -3' miRNA: 3'- -UAGG-CG-GCUaGCUCGcGCUCGgGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 12100 | 0.69 | 0.252695 |
Target: 5'- gGUCUGUaccUGAUCgcgGAGCGCGcGGCCuCGGCc -3' miRNA: 3'- -UAGGCG---GCUAG---CUCGCGC-UCGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14072 | 0.66 | 0.384293 |
Target: 5'- gAUCCGCaGAUCGgcaaagcAGCGCucaaggaagcgGAGaCCGGCg -3' miRNA: 3'- -UAGGCGgCUAGC-------UCGCG-----------CUCgGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14533 | 0.73 | 0.124284 |
Target: 5'- gGUCCGCCGAUgcuuggacgccggcaUGGGCccaGAGCCgGGCg -3' miRNA: 3'- -UAGGCGGCUA---------------GCUCGcg-CUCGGgCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14812 | 0.69 | 0.258958 |
Target: 5'- -aUUGCUGAUUGAGUGuCGAGCCCa-- -3' miRNA: 3'- uaGGCGGCUAGCUCGC-GCUCGGGccg -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 16241 | 0.66 | 0.385144 |
Target: 5'- -cCCGCC--UCGGuGCcaGCGAuGCCUGGCg -3' miRNA: 3'- uaGGCGGcuAGCU-CG--CGCU-CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 16522 | 0.67 | 0.344142 |
Target: 5'- ---aGCgGAUCGGccuGUGCGuGCUCGGCg -3' miRNA: 3'- uaggCGgCUAGCU---CGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 17430 | 0.69 | 0.252695 |
Target: 5'- -cUCGUCGGUCauGAcGCG-GAGCCCGGUg -3' miRNA: 3'- uaGGCGGCUAG--CU-CGCgCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 17719 | 0.73 | 0.126285 |
Target: 5'- -gUCGUCGAuauccUCGGGCcCGAGCUCGGCg -3' miRNA: 3'- uaGGCGGCU-----AGCUCGcGCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 18272 | 0.67 | 0.344142 |
Target: 5'- -aCCGCUGAcggaaUCGGccgacGCGCGGGCaaucgcugccCCGGCc -3' miRNA: 3'- uaGGCGGCU-----AGCU-----CGCGCUCG----------GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 18855 | 0.69 | 0.252695 |
Target: 5'- -gUUGCCGAgaUCGAGgagcugcuCGUGGGCuCCGGCg -3' miRNA: 3'- uaGGCGGCU--AGCUC--------GCGCUCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 19926 | 0.67 | 0.328643 |
Target: 5'- aAUUCGCCGGUCuGAuaGaCGAGCCCaGUc -3' miRNA: 3'- -UAGGCGGCUAG-CUcgC-GCUCGGGcCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 20524 | 0.69 | 0.223236 |
Target: 5'- -cCCGCCGAcCGcaAGCGCccaucAGCgCCGGCa -3' miRNA: 3'- uaGGCGGCUaGC--UCGCGc----UCG-GGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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