Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12398 | 3' | -61 | NC_003324.1 | + | 57390 | 0.69 | 0.276968 |
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Target: 5'- uGCUGgaGGGCUCGgCGGagGCGAg- -3' miRNA: 3'- gCGACggCCCGAGCgGCUagCGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 54424 | 0.7 | 0.251088 |
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Target: 5'- cCGCUGCCGGGcCUCgacugggucauaGCCGGcgGCGAg- -3' miRNA: 3'- -GCGACGGCCC-GAG------------CGGCUagCGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 29849 | 0.7 | 0.238913 |
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Target: 5'- aGCUGCUguGGGCUgUGCCG-UCGUGAcaUCa -3' miRNA: 3'- gCGACGG--CCCGA-GCGGCuAGCGCU--AG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 26555 | 0.71 | 0.22102 |
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Target: 5'- gCGCUGCCGaGGUcgagcaauugaugUCGCCGAcaUUGCcGUCg -3' miRNA: 3'- -GCGACGGC-CCG-------------AGCGGCU--AGCGcUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 35898 | 0.71 | 0.205328 |
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Target: 5'- aGCUGCUGGGCaaGCUGGcUC-CGGUCa -3' miRNA: 3'- gCGACGGCCCGagCGGCU-AGcGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 29261 | 0.72 | 0.175909 |
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Target: 5'- uGCaucGCCGGGCUCGCgcuCGAUCgGCGGa- -3' miRNA: 3'- gCGa--CGGCCCGAGCG---GCUAG-CGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 35392 | 0.73 | 0.146376 |
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Target: 5'- aGCaGCCGGcGCaaucCGCCGG-CGCGAUCg -3' miRNA: 3'- gCGaCGGCC-CGa---GCGGCUaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 49492 | 1.09 | 0.000279 |
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Target: 5'- cCGCUGCCGGGCUCGCCGAUCGCGAUCg -3' miRNA: 3'- -GCGACGGCCCGAGCGGCUAGCGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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