Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12398 | 3' | -61 | NC_003324.1 | + | 35539 | 0.68 | 0.319709 |
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Target: 5'- aCGCUcGCCGacaUCGCCGA-CGUGAUCc -3' miRNA: 3'- -GCGA-CGGCccgAGCGGCUaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 6594 | 0.67 | 0.358148 |
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Target: 5'- -cCUGCCGuGGCUCagcgGCCGAgacgagaUUGCGGUUa -3' miRNA: 3'- gcGACGGC-CCGAG----CGGCU-------AGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 50096 | 0.67 | 0.384086 |
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Target: 5'- aGCgGCCccaGcGCUUGCCGAUCuugugggcgGCGAUCa -3' miRNA: 3'- gCGaCGGc--C-CGAGCGGCUAG---------CGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 42974 | 0.66 | 0.428461 |
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Target: 5'- cCGCcGCUGGG-UCGUucuCGGUCGCaGAUCc -3' miRNA: 3'- -GCGaCGGCCCgAGCG---GCUAGCG-CUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 14516 | 0.66 | 0.437694 |
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Target: 5'- aCGCcgGCaUGGGCccagaGCCGggCGUGAUCc -3' miRNA: 3'- -GCGa-CG-GCCCGag---CGGCuaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 25968 | 0.66 | 0.453647 |
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Target: 5'- -cCUGCaGGGCuuUCuugaaagccucaagGUCGAUCGCGAUCg -3' miRNA: 3'- gcGACGgCCCG--AG--------------CGGCUAGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 12037 | 0.66 | 0.456495 |
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Target: 5'- uGCuUGCCGGGCagggCGCCGAcucuucUC-CGAa- -3' miRNA: 3'- gCG-ACGGCCCGa---GCGGCU------AGcGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 49492 | 1.09 | 0.000279 |
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Target: 5'- cCGCUGCCGGGCUCGCCGAUCGCGAUCg -3' miRNA: 3'- -GCGACGGCCCGAGCGGCUAGCGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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