Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12398 | 3' | -61 | NC_003324.1 | + | 43794 | 0.66 | 0.419344 |
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Target: 5'- gGgUGCCGGGaaCGCCGGcaaggCGCGugaaAUCa -3' miRNA: 3'- gCgACGGCCCgaGCGGCUa----GCGC----UAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 42974 | 0.66 | 0.428461 |
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Target: 5'- cCGCcGCUGGG-UCGUucuCGGUCGCaGAUCc -3' miRNA: 3'- -GCGaCGGCCCgAGCG---GCUAGCG-CUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 14516 | 0.66 | 0.437694 |
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Target: 5'- aCGCcgGCaUGGGCccagaGCCGggCGUGAUCc -3' miRNA: 3'- -GCGa-CG-GCCCGag---CGGCuaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 30048 | 0.66 | 0.437694 |
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Target: 5'- uGCcGCCGcccaucacGC-CGCCGGUCGCGAc- -3' miRNA: 3'- gCGaCGGCc-------CGaGCGGCUAGCGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 28636 | 0.66 | 0.437694 |
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Target: 5'- uCGUUG-CGGGC-CGCCuuGUCGCGcGUCg -3' miRNA: 3'- -GCGACgGCCCGaGCGGc-UAGCGC-UAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 25968 | 0.66 | 0.453647 |
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Target: 5'- -cCUGCaGGGCuuUCuugaaagccucaagGUCGAUCGCGAUCg -3' miRNA: 3'- gcGACGgCCCG--AG--------------CGGCUAGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 12037 | 0.66 | 0.456495 |
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Target: 5'- uGCuUGCCGGGCagggCGCCGAcucuucUC-CGAa- -3' miRNA: 3'- gCG-ACGGCCCGa---GCGGCU------AGcGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 21851 | 0.66 | 0.456495 |
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Target: 5'- gCGCaGCCGGGCcuccUCGgCGGccuuggccuucuUCGgGGUCg -3' miRNA: 3'- -GCGaCGGCCCG----AGCgGCU------------AGCgCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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