Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12398 | 3' | -61 | NC_003324.1 | + | 43794 | 0.66 | 0.419344 |
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Target: 5'- gGgUGCCGGGaaCGCCGGcaaggCGCGugaaAUCa -3' miRNA: 3'- gCgACGGCCCgaGCGGCUa----GCGC----UAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 49106 | 0.67 | 0.358965 |
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Target: 5'- uCGCUGCCGGuGacgcgaUCGCCaaGAUgGCGGg- -3' miRNA: 3'- -GCGACGGCC-Cg-----AGCGG--CUAgCGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 49390 | 0.68 | 0.342867 |
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Target: 5'- aGCgacCCGGGCaaauacUCGAUCGCGAUCg -3' miRNA: 3'- gCGac-GGCCCGagc---GGCUAGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 49492 | 1.09 | 0.000279 |
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Target: 5'- cCGCUGCCGGGCUCGCCGAUCGCGAUCg -3' miRNA: 3'- -GCGACGGCCCGAGCGGCUAGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 50096 | 0.67 | 0.384086 |
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Target: 5'- aGCgGCCccaGcGCUUGCCGAUCuugugggcgGCGAUCa -3' miRNA: 3'- gCGaCGGc--C-CGAGCGGCUAG---------CGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 54424 | 0.7 | 0.251088 |
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Target: 5'- cCGCUGCCGGGcCUCgacugggucauaGCCGGcgGCGAg- -3' miRNA: 3'- -GCGACGGCCC-GAG------------CGGCUagCGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 55321 | 0.67 | 0.400588 |
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Target: 5'- aGCUGCCacagcGGCucaaggaUCGCUGGaaCGCGAUCa -3' miRNA: 3'- gCGACGGc----CCG-------AGCGGCUa-GCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 57390 | 0.69 | 0.276968 |
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Target: 5'- uGCUGgaGGGCUCGgCGGagGCGAg- -3' miRNA: 3'- gCGACggCCCGAGCgGCUagCGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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