Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12398 | 3' | -61 | NC_003324.1 | + | 21851 | 0.66 | 0.456495 |
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Target: 5'- gCGCaGCCGGGCcuccUCGgCGGccuuggccuucuUCGgGGUCg -3' miRNA: 3'- -GCGaCGGCCCG----AGCgGCU------------AGCgCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 19007 | 0.68 | 0.319709 |
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Target: 5'- gGCUGCaCGcGGCUgGCCucGAaCGCGAUg -3' miRNA: 3'- gCGACG-GC-CCGAgCGG--CUaGCGCUAg -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 14539 | 0.67 | 0.36721 |
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Target: 5'- gGC-GCUGGcGCUCaagGCCGA-CGUGGUCa -3' miRNA: 3'- gCGaCGGCC-CGAG---CGGCUaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 14516 | 0.66 | 0.437694 |
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Target: 5'- aCGCcgGCaUGGGCccagaGCCGggCGUGAUCc -3' miRNA: 3'- -GCGa-CG-GCCCGag---CGGCuaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 12037 | 0.66 | 0.456495 |
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Target: 5'- uGCuUGCCGGGCagggCGCCGAcucuucUC-CGAa- -3' miRNA: 3'- gCG-ACGGCCCGa---GCGGCU------AGcGCUag -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 8121 | 0.68 | 0.35085 |
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Target: 5'- uGCUgaaacucacccGCCGGGCagcugcgCGCuCGAcUGCGAUCa -3' miRNA: 3'- gCGA-----------CGGCCCGa------GCG-GCUaGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 6594 | 0.67 | 0.358148 |
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Target: 5'- -cCUGCCGuGGCUCagcgGCCGAgacgagaUUGCGGUUa -3' miRNA: 3'- gcGACGGC-CCGAG----CGGCU-------AGCGCUAG- -5' |
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| 12398 | 3' | -61 | NC_003324.1 | + | 1405 | 0.68 | 0.327296 |
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Target: 5'- aCGgUGCUGGGCguucuucgccUCGUCGAgcagCGCGAc- -3' miRNA: 3'- -GCgACGGCCCG----------AGCGGCUa---GCGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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