Results 21 - 40 of 73 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12408 | 3' | -58.6 | NC_003324.1 | + | 41427 | 0.67 | 0.529954 |
Target: 5'- aAGCCGC-CCC-CAGCG-CCGauuucgaGGCCg -3' miRNA: 3'- gUUGGUGaGGGcGUCGCuGGCg------CUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 54506 | 0.67 | 0.529954 |
Target: 5'- gCAuCCGCgaagCCUGCAgcGCGACCgGCGuuCCg -3' miRNA: 3'- -GUuGGUGa---GGGCGU--CGCUGG-CGCu-GG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 54140 | 0.67 | 0.529954 |
Target: 5'- uCGGCCgAUUCcgCCGCAGCGAaagccuCUGCGcGCCa -3' miRNA: 3'- -GUUGG-UGAG--GGCGUCGCU------GGCGC-UGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 32229 | 0.67 | 0.509545 |
Target: 5'- aCAAuuCCAagCCCGgGGCGGCC-CGGCCc -3' miRNA: 3'- -GUU--GGUgaGGGCgUCGCUGGcGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 16311 | 0.67 | 0.509545 |
Target: 5'- cCAAUUACaagCagcagaCGCAGCGGuCCGCGACg -3' miRNA: 3'- -GUUGGUGa--Gg-----GCGUCGCU-GGCGCUGg -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 56128 | 0.67 | 0.509545 |
Target: 5'- aAGCCgACggCCGUGGCGACUuugGCGAUCu -3' miRNA: 3'- gUUGG-UGagGGCGUCGCUGG---CGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 20568 | 0.67 | 0.509545 |
Target: 5'- uCAAUCGCaacaUCCCGCuccGCGauGCgGCGAUCg -3' miRNA: 3'- -GUUGGUG----AGGGCGu--CGC--UGgCGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 29978 | 0.67 | 0.509545 |
Target: 5'- gCAGCCGggUCCGCAuCGAUCcCGGCCg -3' miRNA: 3'- -GUUGGUgaGGGCGUcGCUGGcGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 40228 | 0.67 | 0.509545 |
Target: 5'- cCGGCUucggugcugGCUCCCGCu-CGACUGCcGCCu -3' miRNA: 3'- -GUUGG---------UGAGGGCGucGCUGGCGcUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 5563 | 0.67 | 0.499464 |
Target: 5'- gCAACC-UUCgCCGCGGgcgucaucggccUGACCGUGAUCg -3' miRNA: 3'- -GUUGGuGAG-GGCGUC------------GCUGGCGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 4487 | 0.67 | 0.499464 |
Target: 5'- gCGGCuCACaUCCUcuacgGCGGCGA-CGCGACUa -3' miRNA: 3'- -GUUG-GUG-AGGG-----CGUCGCUgGCGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 49208 | 0.67 | 0.499464 |
Target: 5'- uCAAUCGagcggCCCGCcaucuuGGCGAUCGCGucACCg -3' miRNA: 3'- -GUUGGUga---GGGCG------UCGCUGGCGC--UGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 54407 | 0.67 | 0.489473 |
Target: 5'- aCGGCaACUCCC-CGG-GACCGCuGCCg -3' miRNA: 3'- -GUUGgUGAGGGcGUCgCUGGCGcUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 45992 | 0.67 | 0.489473 |
Target: 5'- --uUCGCUUCCGaAGCGGCgGCG-CCg -3' miRNA: 3'- guuGGUGAGGGCgUCGCUGgCGCuGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 16133 | 0.67 | 0.479576 |
Target: 5'- aGGgCGCUUCgGUAGCGgggGCCGCG-CCa -3' miRNA: 3'- gUUgGUGAGGgCGUCGC---UGGCGCuGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 8176 | 0.67 | 0.479576 |
Target: 5'- gGGCCAgUCCCuGCucacugcccGGCGGCgCGCG-CCu -3' miRNA: 3'- gUUGGUgAGGG-CG---------UCGCUG-GCGCuGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 6163 | 0.68 | 0.450496 |
Target: 5'- gGACCgACUugCCCuCGGCGAUCuCGACCa -3' miRNA: 3'- gUUGG-UGA--GGGcGUCGCUGGcGCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 21853 | 0.68 | 0.450496 |
Target: 5'- gCAGCCGggcCUCCU-CGGCGGCCuuGGCCu -3' miRNA: 3'- -GUUGGU---GAGGGcGUCGCUGGcgCUGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 12590 | 0.68 | 0.441019 |
Target: 5'- -uGCCACcagCCCGUcguuggacgcguAGCaGACCGCGugACCg -3' miRNA: 3'- guUGGUGa--GGGCG------------UCG-CUGGCGC--UGG- -5' |
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12408 | 3' | -58.6 | NC_003324.1 | + | 14772 | 0.68 | 0.441019 |
Target: 5'- aCGGCUggaaaGCUCagcaggaaaUCGCGGCGAagaaCGCGGCCg -3' miRNA: 3'- -GUUGG-----UGAG---------GGCGUCGCUg---GCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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