Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12416 | 3' | -54.6 | NC_003324.1 | + | 53695 | 0.67 | 0.718346 |
Target: 5'- cGGCGACGuuCGUcGCGcaGCUcGUCGAGUg -3' miRNA: 3'- -CCGUUGUu-GCAaCGC--CGGcCAGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 57378 | 0.66 | 0.799528 |
Target: 5'- cGGCGGaGGCGaggGCGGCaCGaccggcGUUGAGCa -3' miRNA: 3'- -CCGUUgUUGCaa-CGCCG-GC------CAGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 3159 | 0.66 | 0.799528 |
Target: 5'- uGGCAAgAGCGggagcgccgUGCGGCCa-UUGAGa -3' miRNA: 3'- -CCGUUgUUGCa--------ACGCCGGccAGCUCg -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 13170 | 0.67 | 0.749729 |
Target: 5'- aGGCu---GCGUcgGCGG-CGGUCG-GCg -3' miRNA: 3'- -CCGuuguUGCAa-CGCCgGCCAGCuCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 1276 | 0.67 | 0.749729 |
Target: 5'- --uGACGACGUUcggaucGCGGCCGGauuUC-AGCg -3' miRNA: 3'- ccgUUGUUGCAA------CGCCGGCC---AGcUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 45217 | 0.66 | 0.799528 |
Target: 5'- aGGCA-CAuCGUUGUugauacgccGGCagCGGUCGAGa -3' miRNA: 3'- -CCGUuGUuGCAACG---------CCG--GCCAGCUCg -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 22611 | 0.66 | 0.769075 |
Target: 5'- cGGCAAUAACGUcGUGuuccucaCCGGUaccccguUGAGCa -3' miRNA: 3'- -CCGUUGUUGCAaCGCc------GGCCA-------GCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 30614 | 0.66 | 0.75691 |
Target: 5'- cGCGucGCAGCGgcaGCGGCagcgaaggucgccaCGGUCGcaGGCg -3' miRNA: 3'- cCGU--UGUUGCaa-CGCCG--------------GCCAGC--UCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 40496 | 0.67 | 0.725748 |
Target: 5'- aGGCGACGACGaagcGCcagacgaaggcgaaGGCCGGU-GAGg -3' miRNA: 3'- -CCGUUGUUGCaa--CG--------------CCGGCCAgCUCg -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 30088 | 0.68 | 0.686206 |
Target: 5'- cGCAGCAucGCGUcgccagagUGCaccGGCCGGgaUCGAuGCg -3' miRNA: 3'- cCGUUGU--UGCA--------ACG---CCGGCC--AGCU-CG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 20609 | 0.76 | 0.255808 |
Target: 5'- cGGCgcugAugGGCGcUUGCGGUCGG-CGGGCa -3' miRNA: 3'- -CCG----UugUUGC-AACGCCGGCCaGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 15224 | 0.66 | 0.78987 |
Target: 5'- cGGCGGCGagccuuuucACGUgGC-GCCGuGUCGAcGCc -3' miRNA: 3'- -CCGUUGU---------UGCAaCGcCGGC-CAGCU-CG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 29549 | 0.66 | 0.798569 |
Target: 5'- cGCGcccGCAGCcagGCGGCCGugucaucGUCGAGg -3' miRNA: 3'- cCGU---UGUUGcaaCGCCGGC-------CAGCUCg -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 48454 | 0.67 | 0.728906 |
Target: 5'- aGGCGccaaggGCAAC--UGCGaGCCGGcuuggagugCGAGCa -3' miRNA: 3'- -CCGU------UGUUGcaACGC-CGGCCa--------GCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 53291 | 0.67 | 0.728906 |
Target: 5'- cGCAGCuuuCGUUGUGaaGCCGGcCGccAGCu -3' miRNA: 3'- cCGUUGuu-GCAACGC--CGGCCaGC--UCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 54345 | 0.66 | 0.77008 |
Target: 5'- cGGCu-CGGCGcUGauGCCGGUCcauGGCa -3' miRNA: 3'- -CCGuuGUUGCaACgcCGGCCAGc--UCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 40126 | 0.67 | 0.696985 |
Target: 5'- cGCAACuACGgugGaCGGCCGa-CGAGCu -3' miRNA: 3'- cCGUUGuUGCaa-C-GCCGGCcaGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 20563 | 0.68 | 0.675375 |
Target: 5'- cGCAACAucccgcuccGCGaUGCGGC-GaUCGAGCg -3' miRNA: 3'- cCGUUGU---------UGCaACGCCGgCcAGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 35480 | 0.66 | 0.759969 |
Target: 5'- gGGCGAgGAaaccaugcGCGGgCGGcCGAGCa -3' miRNA: 3'- -CCGUUgUUgcaa----CGCCgGCCaGCUCG- -5' |
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12416 | 3' | -54.6 | NC_003324.1 | + | 39953 | 0.67 | 0.728906 |
Target: 5'- cGGCAAgGAa---GCGGCCgacaagaaGGUCGAGg -3' miRNA: 3'- -CCGUUgUUgcaaCGCCGG--------CCAGCUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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