miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12426 3' -58.8 NC_003324.1 + 23153 0.66 0.549677
Target:  5'- cGGCAAaCGUCUUgCCGG-CGCCCA-CGg -3'
miRNA:   3'- -CCGUUgGCAGGG-GGUCaGCGGGUuGC- -5'
12426 3' -58.8 NC_003324.1 + 32896 0.66 0.56008
Target:  5'- aGGCGGCUG-CCCgCCAugCGCCgAACa -3'
miRNA:   3'- -CCGUUGGCaGGG-GGUcaGCGGgUUGc -5'
12426 3' -58.8 NC_003324.1 + 47635 0.66 0.570536
Target:  5'- cGGCGACCGggCCgCCA-UCGUCgAGCu -3'
miRNA:   3'- -CCGUUGGCa-GGgGGUcAGCGGgUUGc -5'
12426 3' -58.8 NC_003324.1 + 43244 0.66 0.570536
Target:  5'- aGGgGGCCG-CCUgCagGGUCGCCUGGCa -3'
miRNA:   3'- -CCgUUGGCaGGGgG--UCAGCGGGUUGc -5'
12426 3' -58.8 NC_003324.1 + 7345 0.66 0.570536
Target:  5'- aGCGACCGUgaUCUCGGUCGaaguuuCCCAGCc -3'
miRNA:   3'- cCGUUGGCAg-GGGGUCAGC------GGGUUGc -5'
12426 3' -58.8 NC_003324.1 + 8168 0.66 0.576833
Target:  5'- cGGCGACCGUggcagccauggucaCCUCCucgacgaAGUUGCCCu-CGu -3'
miRNA:   3'- -CCGUUGGCA--------------GGGGG-------UCAGCGGGuuGC- -5'
12426 3' -58.8 NC_003324.1 + 35883 0.66 0.581039
Target:  5'- uGGCu-CCGgucaaCCCCCAGaUCGCCau-CGc -3'
miRNA:   3'- -CCGuuGGCa----GGGGGUC-AGCGGguuGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.