Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12429 | 3' | -50.1 | NC_003324.1 | + | 29839 | 0.66 | 0.960443 |
Target: 5'- gCCGAUCcuUggGCgCAUcGAGG-CGUa -3' miRNA: 3'- -GGCUAGuuGuuCGgGUAaCUCCaGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 36541 | 0.66 | 0.960443 |
Target: 5'- uCCGAUCuggAACAAGCgCCGc--GGG-CGCg -3' miRNA: 3'- -GGCUAG---UUGUUCG-GGUaacUCCaGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 31864 | 0.66 | 0.960443 |
Target: 5'- gCCGAau-ACGGGCCCGaaGAGGcCGg -3' miRNA: 3'- -GGCUaguUGUUCGGGUaaCUCCaGCg -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 28061 | 0.66 | 0.960443 |
Target: 5'- aUCGAcaucuUCGugAAGCCCGguaccGAGGU-GCu -3' miRNA: 3'- -GGCU-----AGUugUUCGGGUaa---CUCCAgCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 42084 | 0.66 | 0.956389 |
Target: 5'- aCGG-CAACGAGCCUAUcagcaGGGGccgggagcgcuUCGCa -3' miRNA: 3'- gGCUaGUUGUUCGGGUAa----CUCC-----------AGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 24975 | 0.66 | 0.952061 |
Target: 5'- -gGAUgGA-GAGCUUGUUGAGGUgGCg -3' miRNA: 3'- ggCUAgUUgUUCGGGUAACUCCAgCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 57250 | 0.66 | 0.947455 |
Target: 5'- gUGAgCGGCAGGCCC-UUGGcuUCGCa -3' miRNA: 3'- gGCUaGUUGUUCGGGuAACUccAGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 22255 | 0.66 | 0.947455 |
Target: 5'- gCCGAUCGcgGCGAGCUgCGccUUGAuGUCGUc -3' miRNA: 3'- -GGCUAGU--UGUUCGG-GU--AACUcCAGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 2736 | 0.66 | 0.942565 |
Target: 5'- gCCGAUC-GCGcGCCCAcaGuGG-CGCa -3' miRNA: 3'- -GGCUAGuUGUuCGGGUaaCuCCaGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 50687 | 0.66 | 0.942565 |
Target: 5'- aCGAUCuGC-AGCUCGUcGAGGgcggCGUg -3' miRNA: 3'- gGCUAGuUGuUCGGGUAaCUCCa---GCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 22320 | 0.66 | 0.942565 |
Target: 5'- gCGAUCGGCAAGa-CGUUGccgcucccgaAGGUgCGCg -3' miRNA: 3'- gGCUAGUUGUUCggGUAAC----------UCCA-GCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 29151 | 0.66 | 0.942565 |
Target: 5'- aCGAguUCGGCGuGUCCGUcGAGGaccUCGCc -3' miRNA: 3'- gGCU--AGUUGUuCGGGUAaCUCC---AGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 27627 | 0.66 | 0.942565 |
Target: 5'- gCCGG-CAGcCGAGCCCGaaacAGGUcCGCg -3' miRNA: 3'- -GGCUaGUU-GUUCGGGUaac-UCCA-GCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 42507 | 0.67 | 0.937388 |
Target: 5'- uCCGGUCccCAcGGCaaAUUGGGGUCaGCa -3' miRNA: 3'- -GGCUAGuuGU-UCGggUAACUCCAG-CG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 5432 | 0.67 | 0.937388 |
Target: 5'- aUGGUCAGCGucggGGCgCCGUUGGuGUCGa -3' miRNA: 3'- gGCUAGUUGU----UCG-GGUAACUcCAGCg -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 43147 | 0.67 | 0.931923 |
Target: 5'- cUCGA-CGGCGAGCUUGUUGAGcgagCGCc -3' miRNA: 3'- -GGCUaGUUGUUCGGGUAACUCca--GCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 19921 | 0.67 | 0.931923 |
Target: 5'- gCCGGUCugauaGACGAGCCCAgucccUGGGacacaUUGCa -3' miRNA: 3'- -GGCUAG-----UUGUUCGGGUa----ACUCc----AGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 29345 | 0.67 | 0.926167 |
Target: 5'- gCCGAUCGagcGCGAGCCCGgcgaUGcAGGccCGa -3' miRNA: 3'- -GGCUAGU---UGUUCGGGUa---AC-UCCa-GCg -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 11750 | 0.67 | 0.913785 |
Target: 5'- gUGGUCAAUGccguGCCCGgcccGGUCGCg -3' miRNA: 3'- gGCUAGUUGUu---CGGGUaacuCCAGCG- -5' |
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12429 | 3' | -50.1 | NC_003324.1 | + | 32173 | 0.68 | 0.90716 |
Target: 5'- aCCGG-CGACGAGCC----GAGG-CGCa -3' miRNA: 3'- -GGCUaGUUGUUCGGguaaCUCCaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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