Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12432 | 3' | -56.1 | NC_003324.1 | + | 40667 | 0.65 | 0.715399 |
Target: 5'- cCGGACCAcaaccacaucgggcGACAUGaCGACaCGguagUGCCGa -3' miRNA: 3'- -GCCUGGU--------------UUGUAC-GCUGcGCg---ACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 38178 | 0.66 | 0.707973 |
Target: 5'- aCGGucACCAGcACGgcgcCGACGCGCUucGCCa -3' miRNA: 3'- -GCC--UGGUU-UGUac--GCUGCGCGA--CGGc -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 5459 | 0.66 | 0.707973 |
Target: 5'- uGGGUCAGGCAgGCGGCGuCGCccGCCc -3' miRNA: 3'- gCCUGGUUUGUaCGCUGC-GCGa-CGGc -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 45925 | 0.66 | 0.707973 |
Target: 5'- uCGGaaGCgAAGCAaGCGACGCcGCagGCUGg -3' miRNA: 3'- -GCC--UGgUUUGUaCGCUGCG-CGa-CGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 3069 | 0.66 | 0.707973 |
Target: 5'- uGGGCCGcuCGaaGCGACGaCGCaaGCCGa -3' miRNA: 3'- gCCUGGUuuGUa-CGCUGC-GCGa-CGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 19206 | 0.66 | 0.697303 |
Target: 5'- gCGGGCCuGACcgGgauCGACaaGCGCaaUGCCGa -3' miRNA: 3'- -GCCUGGuUUGuaC---GCUG--CGCG--ACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 35718 | 0.66 | 0.686571 |
Target: 5'- cCGaGGCCAAACAgcagcagaugaUGCuGCGCGCagcagaaGCCGa -3' miRNA: 3'- -GC-CUGGUUUGU-----------ACGcUGCGCGa------CGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 27203 | 0.66 | 0.686571 |
Target: 5'- aCGGGCUcaaGAGCGagaGCGAcCGCGCUGgUGu -3' miRNA: 3'- -GCCUGG---UUUGUa--CGCU-GCGCGACgGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 30003 | 0.66 | 0.686571 |
Target: 5'- cCGGugCAcucugGCGACGCGaUGCUGc -3' miRNA: 3'- -GCCugGUuuguaCGCUGCGCgACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 28590 | 0.66 | 0.675788 |
Target: 5'- uCGGuCCGAAgG-GCGccCGCGUUGCCa -3' miRNA: 3'- -GCCuGGUUUgUaCGCu-GCGCGACGGc -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 20907 | 0.66 | 0.675788 |
Target: 5'- uGGACCcgaccgGAACG-GCGAUGCGC-GUCa -3' miRNA: 3'- gCCUGG------UUUGUaCGCUGCGCGaCGGc -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 31093 | 0.66 | 0.664965 |
Target: 5'- cCGGuCCuuGCG-GCaAUGCGCUGCUGc -3' miRNA: 3'- -GCCuGGuuUGUaCGcUGCGCGACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 3373 | 0.66 | 0.663881 |
Target: 5'- gCGGAUCuuuGACAUGUuuaugccGACG-GUUGCCGc -3' miRNA: 3'- -GCCUGGu--UUGUACG-------CUGCgCGACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 9651 | 0.67 | 0.654112 |
Target: 5'- cCGGcaacGCCAAcgcGCuggGCGGCGUGggGCCGg -3' miRNA: 3'- -GCC----UGGUU---UGua-CGCUGCGCgaCGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 17706 | 0.67 | 0.653025 |
Target: 5'- uCGGGcCCGAGCucgGCGGCaucgugcucaacgGCGC-GCCGg -3' miRNA: 3'- -GCCU-GGUUUGua-CGCUG-------------CGCGaCGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 45100 | 0.67 | 0.650852 |
Target: 5'- uGGACCGcgccaucuuguucaGGCucuCGAC-CGCUGCCGg -3' miRNA: 3'- gCCUGGU--------------UUGuacGCUGcGCGACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 23879 | 0.67 | 0.643239 |
Target: 5'- gCGGACCGAGCuccucGaCGAuCGCGUccgcagguuUGCCGu -3' miRNA: 3'- -GCCUGGUUUGua---C-GCU-GCGCG---------ACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 48484 | 0.67 | 0.643239 |
Target: 5'- --cAUCGAGCGcUGCGACGCcGCUGUCc -3' miRNA: 3'- gccUGGUUUGU-ACGCUGCG-CGACGGc -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 18035 | 0.67 | 0.632356 |
Target: 5'- aCGGGCCAuGCGauagGCGGCGag-UGCCGu -3' miRNA: 3'- -GCCUGGUuUGUa---CGCUGCgcgACGGC- -5' |
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12432 | 3' | -56.1 | NC_003324.1 | + | 42380 | 0.67 | 0.631267 |
Target: 5'- cCGGcACguGGCAgGCGGCGCacucagcGUUGCCGa -3' miRNA: 3'- -GCC-UGguUUGUaCGCUGCG-------CGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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