Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12446 | 3' | -53.6 | NC_003324.1 | + | 8738 | 0.72 | 0.430115 |
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Target: 5'- cUUGGCGGCGGUGUCGGaGAaGUCGGu -3' miRNA: 3'- uAGCUGCCGUUACAGCCgCUgUAGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 4619 | 0.72 | 0.430115 |
|
Target: 5'- -aCGgcGCGGCucaucuUGUCGGUGGCGUCGAu -3' miRNA: 3'- uaGC--UGCCGuu----ACAGCCGCUGUAGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 2692 | 0.73 | 0.355947 |
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Target: 5'- uUCGGCaagGGCAcgAUGUCGGCGGCggCAGu -3' miRNA: 3'- uAGCUG---CCGU--UACAGCCGCUGuaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 43413 | 0.74 | 0.347331 |
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Target: 5'- aGUCGugGGUggUcUCGGCGACggCGAa -3' miRNA: 3'- -UAGCugCCGuuAcAGCCGCUGuaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 35461 | 0.75 | 0.269471 |
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Target: 5'- -aCGuCGGCGAUGUCGGCGAgCGUgGGg -3' miRNA: 3'- uaGCuGCCGUUACAGCCGCU-GUAgUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 13164 | 0.77 | 0.211995 |
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Target: 5'- cGUCGGCGGCg--GUCGGCGGCggCGGu -3' miRNA: 3'- -UAGCUGCCGuuaCAGCCGCUGuaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 26453 | 1.04 | 0.002873 |
|
Target: 5'- cAUCGACGGCAAUGUCGGCGACAUCAAu -3' miRNA: 3'- -UAGCUGCCGUUACAGCCGCUGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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