Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12446 | 3' | -53.6 | NC_003324.1 | + | 35461 | 0.75 | 0.269471 |
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Target: 5'- -aCGuCGGCGAUGUCGGCGAgCGUgGGg -3' miRNA: 3'- uaGCuGCCGUUACAGCCGCU-GUAgUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 36405 | 0.66 | 0.776963 |
|
Target: 5'- -aCGAUGGCGcgGUgGaaGACAUCAAc -3' miRNA: 3'- uaGCUGCCGUuaCAgCcgCUGUAGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 39745 | 0.68 | 0.679703 |
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Target: 5'- -aUGGCGGCcuuGGUGUcCGGCGGCAcgugcUCGAu -3' miRNA: 3'- uaGCUGCCG---UUACA-GCCGCUGU-----AGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 43147 | 0.66 | 0.766623 |
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Target: 5'- cUCGACGGCGAgcuUGUUGaGCGAgCGcCAAu -3' miRNA: 3'- uAGCUGCCGUU---ACAGC-CGCU-GUaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 43413 | 0.74 | 0.347331 |
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Target: 5'- aGUCGugGGUggUcUCGGCGACggCGAa -3' miRNA: 3'- -UAGCugCCGuuAcAGCCGCUGuaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 47652 | 0.66 | 0.745519 |
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Target: 5'- cUCGACGGCGAgggucgaUCGGCccuCGUCAu -3' miRNA: 3'- uAGCUGCCGUUac-----AGCCGcu-GUAGUu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 53059 | 0.66 | 0.776963 |
|
Target: 5'- cAUUGGCGGCAggucgcgcucGUcUCGGCG-CGUCAc -3' miRNA: 3'- -UAGCUGCCGU----------UAcAGCCGCuGUAGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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