Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12446 | 3' | -53.6 | NC_003324.1 | + | 8738 | 0.72 | 0.430115 |
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Target: 5'- cUUGGCGGCGGUGUCGGaGAaGUCGGu -3' miRNA: 3'- uAGCUGCCGUUACAGCCgCUgUAGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 7650 | 0.67 | 0.734779 |
|
Target: 5'- aGUCGcgcCGGCAGUGcCGGCuGGCGUUu- -3' miRNA: 3'- -UAGCu--GCCGUUACaGCCG-CUGUAGuu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 5124 | 0.7 | 0.523397 |
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Target: 5'- cUUGGCGGCGGUGUCGauCGAgAUCAc -3' miRNA: 3'- uAGCUGCCGUUACAGCc-GCUgUAGUu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 4619 | 0.72 | 0.430115 |
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Target: 5'- -aCGgcGCGGCucaucuUGUCGGUGGCGUCGAu -3' miRNA: 3'- uaGC--UGCCGuu----ACAGCCGCUGUAGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 2692 | 0.73 | 0.355947 |
|
Target: 5'- uUCGGCaagGGCAcgAUGUCGGCGGCggCAGu -3' miRNA: 3'- uAGCUG---CCGU--UACAGCCGCUGuaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 2498 | 0.66 | 0.791172 |
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Target: 5'- cGUCGGCGGCuucaucaucggcGUUGGCGGuguCAUCAu -3' miRNA: 3'- -UAGCUGCCGuua---------CAGCCGCU---GUAGUu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 1528 | 0.66 | 0.766623 |
|
Target: 5'- -cCGGCGGCAGUGgugcgCGGCGcUAUg-- -3' miRNA: 3'- uaGCUGCCGUUACa----GCCGCuGUAguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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