Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12446 | 3' | -53.6 | NC_003324.1 | + | 31312 | 0.67 | 0.690861 |
Target: 5'- -cCGGCGGCGcUGgcgcuggCGGgGGCAUCGu -3' miRNA: 3'- uaGCUGCCGUuACa------GCCgCUGUAGUu -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 33540 | 0.67 | 0.730452 |
Target: 5'- cGUCGACGGCuccauaaaauUCGGCGAgCGUUAc -3' miRNA: 3'- -UAGCUGCCGuuac------AGCCGCU-GUAGUu -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 47652 | 0.66 | 0.745519 |
Target: 5'- cUCGACGGCGAgggucgaUCGGCccuCGUCAu -3' miRNA: 3'- uAGCUGCCGUUac-----AGCCGcu-GUAGUu -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 53059 | 0.66 | 0.776963 |
Target: 5'- cAUUGGCGGCAggucgcgcucGUcUCGGCG-CGUCAc -3' miRNA: 3'- -UAGCUGCCGU----------UAcAGCCGCuGUAGUu -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 36405 | 0.66 | 0.776963 |
Target: 5'- -aCGAUGGCGcgGUgGaaGACAUCAAc -3' miRNA: 3'- uaGCUGCCGUuaCAgCcgCUGUAGUU- -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 2498 | 0.66 | 0.791172 |
Target: 5'- cGUCGGCGGCuucaucaucggcGUUGGCGGuguCAUCAu -3' miRNA: 3'- -UAGCUGCCGuua---------CAGCCGCU---GUAGUu -5' |
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12446 | 3' | -53.6 | NC_003324.1 | + | 13164 | 0.77 | 0.211995 |
Target: 5'- cGUCGGCGGCg--GUCGGCGGCggCGGu -3' miRNA: 3'- -UAGCUGCCGuuaCAGCCGCUGuaGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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