Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12446 | 5' | -56.1 | NC_003324.1 | + | 57381 | 0.66 | 0.734685 |
Target: 5'- gCUCGGCGGagGCgagggCGGCAcgACCGgCGu -3' miRNA: 3'- gGAGCCGUCg-CGa----GCUGUa-UGGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 54491 | 0.66 | 0.724331 |
Target: 5'- gCC-CGGCAGCGgUCccgggGAguUGCCGUUc -3' miRNA: 3'- -GGaGCCGUCGCgAG-----CUguAUGGCAGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 52987 | 0.66 | 0.70337 |
Target: 5'- --gCGGCGGaacucuaGCUCGACAcguaGCgCGUCGa -3' miRNA: 3'- ggaGCCGUCg------CGAGCUGUa---UG-GCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 51810 | 0.67 | 0.660733 |
Target: 5'- gCUUGGC-GCcCUCGGCgAUugCGUCGn -3' miRNA: 3'- gGAGCCGuCGcGAGCUG-UAugGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 51784 | 0.71 | 0.433145 |
Target: 5'- gCCUCGcugauauccuGCAGgCGCUCGACGcgaaCGUCGg -3' miRNA: 3'- -GGAGC----------CGUC-GCGAGCUGUaug-GCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 50049 | 0.66 | 0.692784 |
Target: 5'- aUCagGGCAGCGCcgaUCGcCAUGCUGaCGa -3' miRNA: 3'- -GGagCCGUCGCG---AGCuGUAUGGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 49724 | 0.69 | 0.54312 |
Target: 5'- -aUCGGCAGCGUcgUCGAaGUugCGaUCGu -3' miRNA: 3'- ggAGCCGUCGCG--AGCUgUAugGC-AGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 49522 | 0.67 | 0.639221 |
Target: 5'- cUCUCGGCcguaucgagcGGCGaggCGACGUGCaugGUCGu -3' miRNA: 3'- -GGAGCCG----------UCGCga-GCUGUAUGg--CAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 47670 | 0.69 | 0.512014 |
Target: 5'- --cCGGU-GCGCUgGACAguuCCGUCGg -3' miRNA: 3'- ggaGCCGuCGCGAgCUGUau-GGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 44101 | 0.79 | 0.137255 |
Target: 5'- aCCUC-GCAGCGCUCGGCAUGaucCCGgCGg -3' miRNA: 3'- -GGAGcCGUCGCGAGCUGUAU---GGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 43602 | 0.67 | 0.671456 |
Target: 5'- aCUUGGCGgucGCGCUCGAC-UGCuCGg-- -3' miRNA: 3'- gGAGCCGU---CGCGAGCUGuAUG-GCagc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 43241 | 0.72 | 0.396446 |
Target: 5'- gCUCGGauuGGCGCUCGcucaACAagcucGCCGUCGa -3' miRNA: 3'- gGAGCCg--UCGCGAGC----UGUa----UGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 41655 | 0.67 | 0.671456 |
Target: 5'- uCgUCGGCGGCGC--GACGUAgCCGaCGc -3' miRNA: 3'- -GgAGCCGUCGCGagCUGUAU-GGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 40266 | 0.68 | 0.58549 |
Target: 5'- uCgUCGGCAuCGCUCGGCugauguucCUGUCGa -3' miRNA: 3'- -GgAGCCGUcGCGAGCUGuau-----GGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 39676 | 0.66 | 0.734685 |
Target: 5'- uCCUCGaCAucGUGCUUGAUGUGCCG-Ca -3' miRNA: 3'- -GGAGCcGU--CGCGAGCUGUAUGGCaGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 39500 | 0.71 | 0.411799 |
Target: 5'- uCgUCGGCAGagaacccggccaaaUGCUCGuACAUcaGCCGUCGu -3' miRNA: 3'- -GgAGCCGUC--------------GCGAGC-UGUA--UGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 38354 | 0.7 | 0.452221 |
Target: 5'- aCCggGGCGGCGCUgGuC--GCCGUCGu -3' miRNA: 3'- -GGagCCGUCGCGAgCuGuaUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 37780 | 0.66 | 0.713889 |
Target: 5'- gCUCGGUcugAGCGCcuaUGACAaacgcACCGUCa -3' miRNA: 3'- gGAGCCG---UCGCGa--GCUGUa----UGGCAGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 33755 | 0.69 | 0.512014 |
Target: 5'- gCCUUGGCGGCaGCgcaGGCGUAUCGagCGu -3' miRNA: 3'- -GGAGCCGUCG-CGag-CUGUAUGGCa-GC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 32403 | 0.7 | 0.481668 |
Target: 5'- aCCUCGGCcGCGCUCu---UACCG-CGc -3' miRNA: 3'- -GGAGCCGuCGCGAGcuguAUGGCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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