Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12446 | 5' | -56.1 | NC_003324.1 | + | 15116 | 0.66 | 0.734685 |
Target: 5'- --aUGGCGuccuGCGCcaugaUCGACcUGCCGUCGc -3' miRNA: 3'- ggaGCCGU----CGCG-----AGCUGuAUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 5126 | 0.66 | 0.692784 |
Target: 5'- uCCUUGGCGGCGgugucgaUCGAgAUcacGCCGUa- -3' miRNA: 3'- -GGAGCCGUCGCg------AGCUgUA---UGGCAgc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 50049 | 0.66 | 0.692784 |
Target: 5'- aUCagGGCAGCGCcgaUCGcCAUGCUGaCGa -3' miRNA: 3'- -GGagCCGUCGCG---AGCuGUAUGGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 23260 | 0.66 | 0.692784 |
Target: 5'- --gCGGUAccGCGCUCGccgacCAUACCGUgGg -3' miRNA: 3'- ggaGCCGU--CGCGAGCu----GUAUGGCAgC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 52987 | 0.66 | 0.70337 |
Target: 5'- --gCGGCGGaacucuaGCUCGACAcguaGCgCGUCGa -3' miRNA: 3'- ggaGCCGUCg------CGAGCUGUa---UG-GCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 37780 | 0.66 | 0.713889 |
Target: 5'- gCUCGGUcugAGCGCcuaUGACAaacgcACCGUCa -3' miRNA: 3'- gGAGCCG---UCGCGa--GCUGUa----UGGCAGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 54491 | 0.66 | 0.724331 |
Target: 5'- gCC-CGGCAGCGgUCccgggGAguUGCCGUUc -3' miRNA: 3'- -GGaGCCGUCGCgAG-----CUguAUGGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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