Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12470 | 3' | -59.2 | NC_003324.1 | + | 52654 | 0.66 | 0.529871 |
Target: 5'- gUCGGCGAUGUUGAcgGUGauagGCUGUCUcauGCu -3' miRNA: 3'- -AGCCGUUGCGACU--CACg---CGGCGGA---CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 57364 | 0.66 | 0.526766 |
Target: 5'- gCGGCAcgaccgGCGUUGAGcaucugaGCgcaauagcaggcugGCCGCUUGCg -3' miRNA: 3'- aGCCGU------UGCGACUCa------CG--------------CGGCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 24096 | 0.66 | 0.526766 |
Target: 5'- aCGGUGGCGCUGAuacccuugucGUaguucugcuccagcGCGCUGaUCUGCg -3' miRNA: 3'- aGCCGUUGCGACU----------CA--------------CGCGGC-GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22685 | 0.66 | 0.51955 |
Target: 5'- cUCGGCAACGCgGcAG-GCagccaaaagGCCGCCg-- -3' miRNA: 3'- -AGCCGUUGCGaC-UCaCG---------CGGCGGacg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 43006 | 0.66 | 0.51955 |
Target: 5'- gUCGGCAACGgaGAaccgGCCgguagugggGCCUGCa -3' miRNA: 3'- -AGCCGUUGCgaCUcacgCGG---------CGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 49837 | 0.66 | 0.516468 |
Target: 5'- aCuGCAGCGUuccacgugaUGguGGUGCGCCcgcugaaccaucccGCCUGCa -3' miRNA: 3'- aGcCGUUGCG---------AC--UCACGCGG--------------CGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 27187 | 0.66 | 0.509308 |
Target: 5'- aUCGGgAACGC-GGGUaaGCGCCuucUCUGCc -3' miRNA: 3'- -AGCCgUUGCGaCUCA--CGCGGc--GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 31102 | 0.66 | 0.506252 |
Target: 5'- gCGGCAauGCGCUGcugcucGGcugcugcuuguucuUGCGCCuggcgcugcagGCCUGCc -3' miRNA: 3'- aGCCGU--UGCGAC------UC--------------ACGCGG-----------CGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 33680 | 0.66 | 0.499153 |
Target: 5'- gUCGGCAACGCgc--UGCGCaaaGCa-GCa -3' miRNA: 3'- -AGCCGUUGCGacucACGCGg--CGgaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 32773 | 0.66 | 0.483095 |
Target: 5'- aCGGCAgugucguuccGCGCaUGAGUGCGgucucuggauggagcCCGCacgcaUGCc -3' miRNA: 3'- aGCCGU----------UGCG-ACUCACGC---------------GGCGg----ACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 49724 | 0.67 | 0.469252 |
Target: 5'- aUCGGCAGCGUcGucgaAGuUGCGaucguuggCGCCUGCc -3' miRNA: 3'- -AGCCGUUGCGaC----UC-ACGCg-------GCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 27949 | 0.67 | 0.469252 |
Target: 5'- cCGGCuACGCcagcGAGgacgaagcgcGCGCCGCCUa- -3' miRNA: 3'- aGCCGuUGCGa---CUCa---------CGCGGCGGAcg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 2797 | 0.67 | 0.469252 |
Target: 5'- aUCGGCuauCGCUugGAGacUGcCGCCGCCg-- -3' miRNA: 3'- -AGCCGuu-GCGA--CUC--AC-GCGGCGGacg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 48755 | 0.67 | 0.459489 |
Target: 5'- -aGGUGAUGCcGAGaUGCGCaGCCaGCg -3' miRNA: 3'- agCCGUUGCGaCUC-ACGCGgCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 38293 | 0.67 | 0.456581 |
Target: 5'- uUCGGCAACuacguguucgacgaGgaGaAGUGCGCCcaaggcauCCUGCa -3' miRNA: 3'- -AGCCGUUG--------------CgaC-UCACGCGGc-------GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22202 | 0.67 | 0.449835 |
Target: 5'- aCGGUGAUGUUG-GUGggcuUGCCGCC-GCg -3' miRNA: 3'- aGCCGUUGCGACuCAC----GCGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 53689 | 0.67 | 0.449835 |
Target: 5'- aCGGCGaaacagGCGCUGGacgcaUGCGaCGCCUGg -3' miRNA: 3'- aGCCGU------UGCGACUc----ACGCgGCGGACg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 20893 | 0.67 | 0.434625 |
Target: 5'- aCGGCGAUGCgcgucaccaacacGGUcCGCCGCCUcGCc -3' miRNA: 3'- aGCCGUUGCGac-----------UCAcGCGGCGGA-CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 9651 | 0.67 | 0.43087 |
Target: 5'- cCGGCAacgccaacGCGCUGGGcgGCGUgggGCCggGCg -3' miRNA: 3'- aGCCGU--------UGCGACUCa-CGCGg--CGGa-CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 24333 | 0.67 | 0.43087 |
Target: 5'- cCGGCAACGCgguccuucGAcGUGUcauucgggaacGCCacaGCCUGCg -3' miRNA: 3'- aGCCGUUGCGa-------CU-CACG-----------CGG---CGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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