Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12470 | 3' | -59.2 | NC_003324.1 | + | 2797 | 0.67 | 0.469252 |
Target: 5'- aUCGGCuauCGCUugGAGacUGcCGCCGCCg-- -3' miRNA: 3'- -AGCCGuu-GCGA--CUC--AC-GCGGCGGacg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 3755 | 0.69 | 0.35997 |
Target: 5'- aUGGC-ACGCU-AGUuCGCgGCCUGCc -3' miRNA: 3'- aGCCGuUGCGAcUCAcGCGgCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 5445 | 0.67 | 0.421564 |
Target: 5'- cUCcGCAucgACGCUGGGUcaggcaggcgGCGUCGCCcGCc -3' miRNA: 3'- -AGcCGU---UGCGACUCA----------CGCGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 9651 | 0.67 | 0.43087 |
Target: 5'- cCGGCAacgccaacGCGCUGGGcgGCGUgggGCCggGCg -3' miRNA: 3'- aGCCGU--------UGCGACUCa-CGCGg--CGGa-CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 10693 | 0.71 | 0.24031 |
Target: 5'- aCGGcCAGCGUUGGGcgacugggggacgguUGcCGuuGCCUGCa -3' miRNA: 3'- aGCC-GUUGCGACUC---------------AC-GCggCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 14684 | 0.68 | 0.39439 |
Target: 5'- cCGGCcgccACGCUaccgccUGCGCCGCCgGCc -3' miRNA: 3'- aGCCGu---UGCGAcuc---ACGCGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 16721 | 0.7 | 0.275823 |
Target: 5'- gUCGauGCAGCGCUGGGcaaucUGCccggcgaguucauGCCGCuCUGCa -3' miRNA: 3'- -AGC--CGUUGCGACUC-----ACG-------------CGGCG-GACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 16882 | 0.74 | 0.152422 |
Target: 5'- gCGGCAAcuauCGUUGAuGUGCGCCGaUUUGCa -3' miRNA: 3'- aGCCGUU----GCGACU-CACGCGGC-GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 16996 | 0.72 | 0.226048 |
Target: 5'- aUCGGUGACGCUGGca--GCCGCCaccuUGCa -3' miRNA: 3'- -AGCCGUUGCGACUcacgCGGCGG----ACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 17976 | 0.68 | 0.368376 |
Target: 5'- -aGGUcGCGCUG-GUGUuCCGCCaGCa -3' miRNA: 3'- agCCGuUGCGACuCACGcGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 20893 | 0.67 | 0.434625 |
Target: 5'- aCGGCGAUGCgcgucaccaacacGGUcCGCCGCCUcGCc -3' miRNA: 3'- aGCCGUUGCGac-----------UCAcGCGGCGGA-CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 21066 | 0.7 | 0.297627 |
Target: 5'- cCGGCaAACGCUGccuUGUcgGCCGCCgcgGCg -3' miRNA: 3'- aGCCG-UUGCGACuc-ACG--CGGCGGa--CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22202 | 0.67 | 0.449835 |
Target: 5'- aCGGUGAUGUUG-GUGggcuUGCCGCC-GCg -3' miRNA: 3'- aGCCGUUGCGACuCAC----GCGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22317 | 0.69 | 0.35997 |
Target: 5'- aUCGGCAagACGUUGccgcucccgaaGGUGCGCgGCg-GCa -3' miRNA: 3'- -AGCCGU--UGCGAC-----------UCACGCGgCGgaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22685 | 0.66 | 0.51955 |
Target: 5'- cUCGGCAACGCgGcAG-GCagccaaaagGCCGCCg-- -3' miRNA: 3'- -AGCCGUUGCGaC-UCaCG---------CGGCGGacg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 24096 | 0.66 | 0.526766 |
Target: 5'- aCGGUGGCGCUGAuacccuugucGUaguucugcuccagcGCGCUGaUCUGCg -3' miRNA: 3'- aGCCGUUGCGACU----------CA--------------CGCGGC-GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 24333 | 0.67 | 0.43087 |
Target: 5'- cCGGCAACGCgguccuucGAcGUGUcauucgggaacGCCacaGCCUGCg -3' miRNA: 3'- aGCCGUUGCGa-------CU-CACG-----------CGG---CGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 24431 | 0.73 | 0.174168 |
Target: 5'- -gGGCGAUGCgaacauUGCGCCGaCCUGCa -3' miRNA: 3'- agCCGUUGCGacuc--ACGCGGC-GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 26826 | 0.74 | 0.152422 |
Target: 5'- uUUGGCGccgcucCGCUGAGUGCcucgucgaucGCCGCCaUGCc -3' miRNA: 3'- -AGCCGUu-----GCGACUCACG----------CGGCGG-ACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 27187 | 0.66 | 0.509308 |
Target: 5'- aUCGGgAACGC-GGGUaaGCGCCuucUCUGCc -3' miRNA: 3'- -AGCCgUUGCGaCUCA--CGCGGc--GGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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