Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12470 | 3' | -59.2 | NC_003324.1 | + | 14684 | 0.68 | 0.39439 |
Target: 5'- cCGGCcgccACGCUaccgccUGCGCCGCCgGCc -3' miRNA: 3'- aGCCGu---UGCGAcuc---ACGCGGCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 48703 | 0.68 | 0.368376 |
Target: 5'- gCGGCGGaGCcgGAugcggGCGCCGaCCUGCg -3' miRNA: 3'- aGCCGUUgCGa-CUca---CGCGGC-GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 31312 | 0.68 | 0.376916 |
Target: 5'- cCGGCGGCGCUG---GCGCUGgCggggGCa -3' miRNA: 3'- aGCCGUUGCGACucaCGCGGCgGa---CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 57308 | 0.68 | 0.368376 |
Target: 5'- cUUGGCGAaGUUcuuGUGCGCCaGCUUGCa -3' miRNA: 3'- -AGCCGUUgCGAcu-CACGCGG-CGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 22317 | 0.69 | 0.35997 |
Target: 5'- aUCGGCAagACGUUGccgcucccgaaGGUGCGCgGCg-GCa -3' miRNA: 3'- -AGCCGU--UGCGAC-----------UCACGCGgCGgaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 38104 | 0.7 | 0.297627 |
Target: 5'- gUCGGCGgaAUGUUGAcgcgccccGUGCGCCGCaaccGCu -3' miRNA: 3'- -AGCCGU--UGCGACU--------CACGCGGCGga--CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 45443 | 0.71 | 0.2566 |
Target: 5'- -gGGCcuCGCUGcu--CGCCGCCUGCu -3' miRNA: 3'- agCCGuuGCGACucacGCGGCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 10693 | 0.71 | 0.24031 |
Target: 5'- aCGGcCAGCGUUGGGcgacugggggacgguUGcCGuuGCCUGCa -3' miRNA: 3'- aGCC-GUUGCGACUC---------------AC-GCggCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 16996 | 0.72 | 0.226048 |
Target: 5'- aUCGGUGACGCUGGca--GCCGCCaccuUGCa -3' miRNA: 3'- -AGCCGUUGCGACUcacgCGGCGG----ACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 3755 | 0.69 | 0.35997 |
Target: 5'- aUGGC-ACGCU-AGUuCGCgGCCUGCc -3' miRNA: 3'- aGCCGuUGCGAcUCAcGCGgCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 50622 | 0.68 | 0.385587 |
Target: 5'- cUCGGCGucgagcaggucaACGC-GGGUGCugGCUGCgUGCc -3' miRNA: 3'- -AGCCGU------------UGCGaCUCACG--CGGCGgACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 30452 | 0.67 | 0.421564 |
Target: 5'- gCGGCAugGCcGAGgGCGaCGCCaucaagGCu -3' miRNA: 3'- aGCCGUugCGaCUCaCGCgGCGGa-----CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 20893 | 0.67 | 0.434625 |
Target: 5'- aCGGCGAUGCgcgucaccaacacGGUcCGCCGCCUcGCc -3' miRNA: 3'- aGCCGUUGCGac-----------UCAcGCGGCGGA-CG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 38293 | 0.67 | 0.456581 |
Target: 5'- uUCGGCAACuacguguucgacgaGgaGaAGUGCGCCcaaggcauCCUGCa -3' miRNA: 3'- -AGCCGUUG--------------CgaC-UCACGCGGc-------GGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 48755 | 0.67 | 0.459489 |
Target: 5'- -aGGUGAUGCcGAGaUGCGCaGCCaGCg -3' miRNA: 3'- agCCGUUGCGaCUC-ACGCGgCGGaCG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 49724 | 0.67 | 0.469252 |
Target: 5'- aUCGGCAGCGUcGucgaAGuUGCGaucguuggCGCCUGCc -3' miRNA: 3'- -AGCCGUUGCGaC----UC-ACGCg-------GCGGACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 27949 | 0.67 | 0.469252 |
Target: 5'- cCGGCuACGCcagcGAGgacgaagcgcGCGCCGCCUa- -3' miRNA: 3'- aGCCGuUGCGa---CUCa---------CGCGGCGGAcg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 2797 | 0.67 | 0.469252 |
Target: 5'- aUCGGCuauCGCUugGAGacUGcCGCCGCCg-- -3' miRNA: 3'- -AGCCGuu-GCGA--CUC--AC-GCGGCGGacg -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 32773 | 0.66 | 0.483095 |
Target: 5'- aCGGCAgugucguuccGCGCaUGAGUGCGgucucuggauggagcCCGCacgcaUGCc -3' miRNA: 3'- aGCCGU----------UGCG-ACUCACGC---------------GGCGg----ACG- -5' |
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12470 | 3' | -59.2 | NC_003324.1 | + | 33680 | 0.66 | 0.499153 |
Target: 5'- gUCGGCAACGCgc--UGCGCaaaGCa-GCa -3' miRNA: 3'- -AGCCGUUGCGacucACGCGg--CGgaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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