Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12479 | 3' | -58.2 | NC_003324.1 | + | 35107 | 0.66 | 0.593124 |
Target: 5'- -uCUGCCUuucCGUCGucGccAGCGCCGuCGCc -3' miRNA: 3'- guGGCGGA---GUAGCuuC--UCGCGGC-GCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 21144 | 0.66 | 0.593124 |
Target: 5'- -uCgGCCUCGUCaguGAGCGagcgggCGCGCg -3' miRNA: 3'- guGgCGGAGUAGcuuCUCGCg-----GCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 282 | 0.66 | 0.58246 |
Target: 5'- uGCUGCUgugaccacUCAcaGGAGAGCGCUaugGCGCg -3' miRNA: 3'- gUGGCGG--------AGUagCUUCUCGCGG---CGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 20688 | 0.66 | 0.58246 |
Target: 5'- aGCCGUCUCuUCGAGcucCGuuGCGCc -3' miRNA: 3'- gUGGCGGAGuAGCUUcucGCggCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 34144 | 0.66 | 0.571834 |
Target: 5'- aGCCGCUggCAUU---GGGCGCCGUGg -3' miRNA: 3'- gUGGCGGa-GUAGcuuCUCGCGGCGCg -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 6255 | 0.66 | 0.571834 |
Target: 5'- gGCUGCCUCucgcauGUCGucGGGCagcgcauuccagGCgGCGCg -3' miRNA: 3'- gUGGCGGAG------UAGCuuCUCG------------CGgCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 42729 | 0.66 | 0.571834 |
Target: 5'- aAUgGCCUUgcuGUUGcAGAuccgcgcccgGCGCCGCGCg -3' miRNA: 3'- gUGgCGGAG---UAGCuUCU----------CGCGGCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 18489 | 0.66 | 0.571834 |
Target: 5'- cUugCGagCUCGUUGgcGAGCGUCacgGCGCg -3' miRNA: 3'- -GugGCg-GAGUAGCuuCUCGCGG---CGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 31008 | 0.66 | 0.571834 |
Target: 5'- -uUCGCuCUCGUCGAucgcuuGGuAGCGaCCgGCGCg -3' miRNA: 3'- guGGCG-GAGUAGCU------UC-UCGC-GG-CGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 39875 | 0.66 | 0.570773 |
Target: 5'- gAUCGUCgUCAUCGAGG-GCGaggugcaCCGUGCc -3' miRNA: 3'- gUGGCGG-AGUAGCUUCuCGC-------GGCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 55985 | 0.66 | 0.570773 |
Target: 5'- aCugCGCgcgaUCGUCGAgaagcgcacccacAGGGCGgaGCGCg -3' miRNA: 3'- -GugGCGg---AGUAGCU-------------UCUCGCggCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 20783 | 0.66 | 0.561255 |
Target: 5'- cCACCGCCggaaCGUUGgcGAG-GCgGCGg -3' miRNA: 3'- -GUGGCGGa---GUAGCuuCUCgCGgCGCg -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 29308 | 0.66 | 0.561255 |
Target: 5'- aGCCGCCgUC-UUGguGAGCGCaccguaGUGCu -3' miRNA: 3'- gUGGCGG-AGuAGCuuCUCGCGg-----CGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 40600 | 0.66 | 0.561255 |
Target: 5'- uCGCCGCgCUCgAUCGuggcuccaucGGGCGUgGUGCg -3' miRNA: 3'- -GUGGCG-GAG-UAGCuu--------CUCGCGgCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 36840 | 0.66 | 0.55073 |
Target: 5'- cUAUCGcCCUCGUCGA----UGCUGCGCu -3' miRNA: 3'- -GUGGC-GGAGUAGCUucucGCGGCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 35858 | 0.66 | 0.55073 |
Target: 5'- cCugCGCCUgG-CGAAcAGUGgCGCGCc -3' miRNA: 3'- -GugGCGGAgUaGCUUcUCGCgGCGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 47837 | 0.66 | 0.55073 |
Target: 5'- --aCGCCUUAU-GAGGcGGCGCUGcCGCu -3' miRNA: 3'- gugGCGGAGUAgCUUC-UCGCGGC-GCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 17264 | 0.67 | 0.540266 |
Target: 5'- aGCCGCgUC--CGAcAGAcGCGCCaGCGCc -3' miRNA: 3'- gUGGCGgAGuaGCU-UCU-CGCGG-CGCG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 17030 | 0.67 | 0.540266 |
Target: 5'- -cCCGCgUC-UUGAucGAGCGCUGCaGCg -3' miRNA: 3'- guGGCGgAGuAGCUu-CUCGCGGCG-CG- -5' |
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12479 | 3' | -58.2 | NC_003324.1 | + | 33427 | 0.67 | 0.540266 |
Target: 5'- aGCCGCUUCAUCGgcGuuGU-CCGUGUa -3' miRNA: 3'- gUGGCGGAGUAGCuuCu-CGcGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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