Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12493 | 5' | -53.8 | NC_003324.1 | + | 17114 | 0.83 | 0.097273 |
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Target: 5'- -aCGCUGcAGCGCucGAUCAAGACGCGGg -3' miRNA: 3'- uaGCGACcUUGCG--CUAGUUCUGCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 17039 | 0.66 | 0.783494 |
|
Target: 5'- -gCGCUGGGuaaggaacuACGCGAUCugcugGCGGa -3' miRNA: 3'- uaGCGACCU---------UGCGCUAGuucugCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 16432 | 0.7 | 0.58968 |
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Target: 5'- gGUC-CUGGAcgACGCGAUCGAcggcaacgccGuCGCGGa -3' miRNA: 3'- -UAGcGACCU--UGCGCUAGUU----------CuGCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 15665 | 0.71 | 0.493232 |
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Target: 5'- -gCGCUcGAugACGCGAUCAAGGCG-GGc -3' miRNA: 3'- uaGCGAcCU--UGCGCUAGUUCUGCgCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 14540 | 0.69 | 0.633813 |
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Target: 5'- -gCGCUGGcgcucaaggccGACGUGGUCAacauGGGuCGCGGc -3' miRNA: 3'- uaGCGACC-----------UUGCGCUAGU----UCU-GCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 7767 | 0.66 | 0.803075 |
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Target: 5'- cUCGCagGGAgGCGCGAUCGcaacGGGCuuugccgccGCGGc -3' miRNA: 3'- uAGCGa-CCU-UGCGCUAGU----UCUG---------CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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