Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12617 | 3' | -56.6 | NC_003345.1 | + | 7170 | 0.71 | 0.46022 |
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Target: 5'- uUCGGUCGGGcuaucACGUCGUgGGUCUUgGUa -3' miRNA: 3'- -AGCCAGCUU-----UGCGGCAgUCGGAGgCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 53114 | 0.72 | 0.42298 |
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Target: 5'- aCGGUCGucuCGCCGUCAaUCUCCu- -3' miRNA: 3'- aGCCAGCuuuGCGGCAGUcGGAGGcg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 4738 | 0.72 | 0.42298 |
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Target: 5'- cUCGGUCGAuuGCGuuGaaCGGCCUuCCGUc -3' miRNA: 3'- -AGCCAGCUu-UGCggCa-GUCGGA-GGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 21340 | 0.76 | 0.241653 |
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Target: 5'- uUCGGUCGGAGCGCCGUU-GUCgUCgGUg -3' miRNA: 3'- -AGCCAGCUUUGCGGCAGuCGG-AGgCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 70274 | 0.79 | 0.169123 |
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Target: 5'- -aGGUCGGcAAgGUCGUCAGCCUCCa- -3' miRNA: 3'- agCCAGCU-UUgCGGCAGUCGGAGGcg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 31130 | 1.12 | 0.000876 |
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Target: 5'- uUCGGUCGAAACGCCGUCAGCCUCCGCu -3' miRNA: 3'- -AGCCAGCUUUGCGGCAGUCGGAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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