Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13110 | 5' | -62.5 | NC_003401.1 | + | 96039 | 0.66 | 0.580864 |
Target: 5'- gGCCAaC-UCACUaACCGGUCUCCUUUGg -3' miRNA: 3'- -CGGUcGcGGUGG-UGGCCGGAGGAGAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 27972 | 0.66 | 0.548645 |
Target: 5'- -aCAGCGCCAUgacguccuuguguaUGCCGaCCUCCUCg- -3' miRNA: 3'- cgGUCGCGGUG--------------GUGGCcGGAGGAGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 118607 | 0.67 | 0.541879 |
Target: 5'- cCCcGCGaucgCACCuuugGCCGGCCggCCUCUGc -3' miRNA: 3'- cGGuCGCg---GUGG----UGGCCGGa-GGAGAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 118850 | 0.67 | 0.541879 |
Target: 5'- cCCcGUGCgCAUCGCCGcCCUCCUCg- -3' miRNA: 3'- cGGuCGCG-GUGGUGGCcGGAGGAGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 12340 | 0.67 | 0.532262 |
Target: 5'- cGCCAGC-CCACCACCgaaacgcgGGUCUUCg--- -3' miRNA: 3'- -CGGUCGcGGUGGUGG--------CCGGAGGagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 41181 | 0.67 | 0.531303 |
Target: 5'- aGCCAcgGCCGCCGCUcugucaaacugcaGGCCUCUgCUGu -3' miRNA: 3'- -CGGUcgCGGUGGUGG-------------CCGGAGGaGAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 17643 | 0.67 | 0.513218 |
Target: 5'- cGCCGcguccGCGUCGCCGCCGGaaUCCa--- -3' miRNA: 3'- -CGGU-----CGCGGUGGUGGCCggAGGagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 132657 | 0.67 | 0.513218 |
Target: 5'- gGCgCGGCGCCGCCGggcUCGGCCggaCCg--- -3' miRNA: 3'- -CG-GUCGCGGUGGU---GGCCGGa--GGagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 119951 | 0.67 | 0.513218 |
Target: 5'- uGCCGcuGCGCCGCCuCCGGCCa------ -3' miRNA: 3'- -CGGU--CGCGGUGGuGGCCGGaggagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 8753 | 0.67 | 0.513218 |
Target: 5'- aGCCGGuCGCCGCCACagGGCg-CCUg-- -3' miRNA: 3'- -CGGUC-GCGGUGGUGg-CCGgaGGAgac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 132347 | 0.67 | 0.510385 |
Target: 5'- cGCCGGCccgggcccgcggccGCCGCCcCCGGCCgCCcCg- -3' miRNA: 3'- -CGGUCG--------------CGGUGGuGGCCGGaGGaGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 120368 | 0.67 | 0.503801 |
Target: 5'- uGCUAGCGUUuucCCACUGGCCguuaguaUUCUGg -3' miRNA: 3'- -CGGUCGCGGu--GGUGGCCGGag-----GAGAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 128043 | 0.67 | 0.503801 |
Target: 5'- uGCCAGCaGCUggcGCgCACCGGCacgccugUCCUCg- -3' miRNA: 3'- -CGGUCG-CGG---UG-GUGGCCGg------AGGAGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 121947 | 0.67 | 0.500991 |
Target: 5'- gGCUGGCGCgGCCgugccacgcccccuGCCGcGCCUCCcggcCUGu -3' miRNA: 3'- -CGGUCGCGgUGG--------------UGGC-CGGAGGa---GAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 27687 | 0.68 | 0.485198 |
Target: 5'- gGCCAGCG-CACaGCCGGCCcCCg--- -3' miRNA: 3'- -CGGUCGCgGUGgUGGCCGGaGGagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 9368 | 0.69 | 0.422892 |
Target: 5'- cGCCGGCGCC-CUACUGGCCg------ -3' miRNA: 3'- -CGGUCGCGGuGGUGGCCGGaggagac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 119527 | 0.69 | 0.414387 |
Target: 5'- cGCCGGCcggucaaaaaGCCACaGCaCGGCCU-CUCUGu -3' miRNA: 3'- -CGGUCG----------CGGUGgUG-GCCGGAgGAGAC- -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 132825 | 0.69 | 0.414387 |
Target: 5'- cGCCgaGGCGCCccggGCC-CCGGCCccCCUCc- -3' miRNA: 3'- -CGG--UCGCGG----UGGuGGCCGGa-GGAGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 89844 | 0.69 | 0.414387 |
Target: 5'- aGCCAGgGaCGCC-CCuGCCUCCUCc- -3' miRNA: 3'- -CGGUCgCgGUGGuGGcCGGAGGAGac -5' |
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13110 | 5' | -62.5 | NC_003401.1 | + | 48842 | 0.69 | 0.405987 |
Target: 5'- -aCAGCGCCGCCuuuACCcaaGCgCUCCUCUc -3' miRNA: 3'- cgGUCGCGGUGG---UGGc--CG-GAGGAGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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