Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13283 | 3' | -55 | NC_003409.1 | + | 86610 | 0.66 | 0.934258 |
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Target: 5'- uGCGGGGaccuGGACCUcggGCGc-CGCCUAUCCg -3' miRNA: 3'- -UGCCUC----CUUGGA---UGCauGUGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 52387 | 0.66 | 0.937696 |
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Target: 5'- uCGGAGGcuaguGCCUggauaagagcugccACGU-C-CCCGCCCc -3' miRNA: 3'- uGCCUCCu----UGGA--------------UGCAuGuGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 30107 | 0.66 | 0.939133 |
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Target: 5'- gACGGuGGGGCaaAUGUGuCACCaagCGCCCc -3' miRNA: 3'- -UGCCuCCUUGgaUGCAU-GUGG---GUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 133740 | 0.66 | 0.939133 |
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Target: 5'- gGCGuucGGGAuCCacggaGCGUACACCCACguCCa -3' miRNA: 3'- -UGCc--UCCUuGGa----UGCAUGUGGGUG--GG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 15688 | 0.66 | 0.939133 |
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Target: 5'- -gGGAGGggUCU--GUACA-CCACCUu -3' miRNA: 3'- ugCCUCCuuGGAugCAUGUgGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 51929 | 0.66 | 0.939133 |
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Target: 5'- -aGGcuccGGGAACCgUGCGUggagaaagccuAgACCCGCCUg -3' miRNA: 3'- ugCC----UCCUUGG-AUGCA-----------UgUGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 109859 | 0.66 | 0.939133 |
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Target: 5'- gGCGGuGGcGCCcgGCGcaacACugCCACCg -3' miRNA: 3'- -UGCCuCCuUGGa-UGCa---UGugGGUGGg -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 70218 | 0.65 | 0.94377 |
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Target: 5'- cGCGGGGGucCCgaguaGUGCACgUCGCCa -3' miRNA: 3'- -UGCCUCCuuGGaug--CAUGUG-GGUGGg -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 65054 | 0.65 | 0.94377 |
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Target: 5'- aGCGG-GGAaaagcACCaGCGU--AUCCGCCCu -3' miRNA: 3'- -UGCCuCCU-----UGGaUGCAugUGGGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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