Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13283 | 3' | -55 | NC_003409.1 | + | 52154 | 0.74 | 0.559393 |
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Target: 5'- cACGGGGGGugGCCUgugGCGUgugcGCGCUgGCCCc -3' miRNA: 3'- -UGCCUCCU--UGGA---UGCA----UGUGGgUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 131696 | 0.72 | 0.661594 |
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Target: 5'- gGCGGccAGGGACuaccgCUGCGUggcACACCCACUg -3' miRNA: 3'- -UGCC--UCCUUG-----GAUGCA---UGUGGGUGGg -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 114579 | 0.74 | 0.569498 |
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Target: 5'- uGCGGgugucgcgcuuuGGGGGCCUGCcaGC-CCCACCCc -3' miRNA: 3'- -UGCC------------UCCUUGGAUGcaUGuGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 34521 | 0.72 | 0.671816 |
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Target: 5'- ---cAGGGACCUGCGcgGCgagcucauGCCCACCCu -3' miRNA: 3'- ugccUCCUUGGAUGCa-UG--------UGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 133345 | 0.71 | 0.73215 |
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Target: 5'- cACGGAuGGACCgg---ACACCUACCCu -3' miRNA: 3'- -UGCCUcCUUGGaugcaUGUGGGUGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 25901 | 0.7 | 0.741954 |
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Target: 5'- -aGGGGGAcagcgcguaccgACCUAUGcGCGCCCACa- -3' miRNA: 3'- ugCCUCCU------------UGGAUGCaUGUGGGUGgg -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 118578 | 0.69 | 0.798427 |
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Target: 5'- cGCGGcgcaccccAGGAACCcgGCGcggcGCACCCaggaACCCg -3' miRNA: 3'- -UGCC--------UCCUUGGa-UGCa---UGUGGG----UGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 23355 | 0.69 | 0.816136 |
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Target: 5'- cUGGAGGuGACCc-CGUGCACCCgggcGCUCu -3' miRNA: 3'- uGCCUCC-UUGGauGCAUGUGGG----UGGG- -5' |
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| 13283 | 3' | -55 | NC_003409.1 | + | 30107 | 0.66 | 0.939133 |
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Target: 5'- gACGGuGGGGCaaAUGUGuCACCaagCGCCCc -3' miRNA: 3'- -UGCCuCCUUGgaUGCAU-GUGG---GUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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